杂种杨树木葡聚糖内糖基转移酶基因（XET）的生物信息学分析

目的]通过生物信息学方法对杂种杨树木葡聚糖内糖基转移酶基因的DNA序列、蛋白质序列进行分析预测,为进一步研究奠定基础.方法]以XET基因及其氨基酸序列为研究对象,利用多种网络数据库及生物信息学软件对其进行分析预测.结果]该基因编码290个氨基酸残基,相对分子质量33 672.0 D,理论等电点（pI）7.04;该酶可能存在3个卷曲结构部位、1个信号肽剪切位点和1个跨膜区,是典型的分泌蛋白;三级结构同源建模预测结果较好,二级结构预测中的螺旋、卷曲和折叠均可见.相似性比对和进化分析显示其与葡萄等的序列相似度较高;而蛋白质三级结构的预测则发现,该酶与几种杆菌的蛋白较接近.结论]XET基因可能在很多生物中具有较高的保守性.

Bioinformatics Analysis of Populus alba × Populus tremula var. glandulosa Xyloglucan Endotransglycosylase (XET) Gene

Objective] Analyze and predict the structure and feature of Populus alba × Populus tremula var.glandulosa xyloglucan endotransglycosylase （XET） gene/protein sequence through bioinformatics method,and pave the way for the downstream experiments.Method] Utilize various online databases and bioinformatics software to predict XET gene and its corresponding protein.Result] XET gene encodes 290 aa; protein molecular weight is 33 672.0 D,theoretical isoelectric point is 7.04 ; XET contains 3 coiled coils,1 signal peptide and 1 transmembrane domain indicating that it is a typical secretory protein; prediction of secondary structure and homologous modeling of tertiary structure both returned rational results.Conclusion] It is possible that XET gene possesses high conservation in different species.