| 正交试验设计优化马粪海胆生殖腺粗脂肪提取工艺研究 |
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| 引用本文： | 王洪英,马娜娜,李梦鑫,孟竹,尚彦琪,张海雷,薛晓宇,郭承华.正交试验设计优化马粪海胆生殖腺粗脂肪提取工艺研究[J].安徽农业科学,2013(27):11064-11065. |
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| 作者姓名： | 王洪英 马娜娜 李梦鑫 孟竹 尚彦琪 张海雷 薛晓宇 郭承华 |
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| 作者单位： | 烟台大学生命科学学院,山东烟台,264005;烟台大学生命科学学院,山东烟台,264005;烟台大学生命科学学院,山东烟台,264005;烟台大学生命科学学院,山东烟台,264005;烟台大学生命科学学院,山东烟台,264005;烟台大学生命科学学院,山东烟台,264005;烟台大学生命科学学院,山东烟台,264005;烟台大学生命科学学院,山东烟台,264005 |
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| 基金项目： | 山东省自然科学基金(ZR2013DM002);烟台大学大学生科技创新基金立项资助项目(121708) |
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| 摘 要： | 目的]以马粪海胆生殖腺为材料，通过正交设计优选提取粗脂肪的最佳工艺条件。方法]采用索氏提取法提取马粪海胆生殖腺粗脂肪。通过单因素试验和正交试验法优选提取马粪海胆生殖腺粗脂肪的最佳工艺条件。结果]提取马粪海胆生殖腺粗脂肪的最佳工艺条件为：提取时间6h，提取温度70℃，料液比1：80，提取次数2次。结论]在此优化条件下马粪海胆生殖腺的粗脂肪得率为17．4％。
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| 关 键 词： | 马粪海胆 生殖腺 粗脂肪 索氏提取法 正交实验法 |
Optimization of Extraction Process of Rude Fat from the Gonad of Hemicentrous pulcherrimus by Orthogonal Experiment Design |
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| Institution: | WANG Hong-ying et al ( College of Life Sciences, Yantai University, Yantai, Shandong 264005 ) |
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| Abstract: | Objective ] The gonad from Hemicentrous pulcherrimus were used as experimental materials. The optimal extraction process of rude fat was obtained by orthogonal experiment design. Method] The rude fat from the gonad of H. pulcherrimus was extracted with Soxhlet extraction method. The optimal extraction process of rude fat was obtained by single-factor experiment and oI~laogonal experiment design. Result] The op- timal extraction process of rude fat： the ratio of material to water was 1： ：80, extracting time for 6 hours, extracting 2 times, at the temperature of 70 ℃. Conclusions] Under the above optimal conditions, the yield of rude fat could reach to 17.4%. |
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| Keywords: | Hemicentrous pulcherrimus Gonad Rude fat Soxhlet extraction method Orthognnal experiment |
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