高羊茅成熟胚愈伤组织诱导及植株再生

目的]优化高羊茅成熟胚的组织培养条件。方法]以高羊茅成熟胚为外植体,MS为基本培养基,探讨不同浓度激素和激素组合对愈伤组织诱导、继代培养及植株分化的影响。结果]添加2,4-D2.0mg/L的MS培养基中愈伤组织的诱导率最高,达63.5%;在1.0～4.0mg/L范围内,随着培养基中2,4-D浓度的降低,愈伤组织的颗粒性增强,致密性增加,含水量下降,其中MS＋2,4-D2.0mg/L为愈伤组织继代的最佳培养基;愈伤组织在含2.0mg/L6-BA和0.10mg/LNAA的MS培养基上植株分化率最高,达80.2%;将再生苗转移至不含植物生长调节剂的1/2MS培养基上进行增殖、壮苗,然后将其移栽入沙质壤土中,移栽成活率可达90%。结论]该研究建立了高羊茅的高效再生体系。

Callus Induction and Plantlet Regeneration from Mature Embryoes of Festuca arundinacea

Objective] The study was to optimize the tissue culture conditions of Festuca arundinacea mature embryoes.Methods] With F.arundinacea mature embryos as the explants and MS as the basic medium.The effects of different concentrations of hormones and their combinations on callus induction,subculture and plant differentiation were studied.Results] The callus induction rate of the explants in MS medium with 2,4-D 2.0 mg/L was the highest,reaching 63.5%.In the range of 1.0-4.0 mg/L,with the decrease of 2,4-D concentration in the medium the graininess and compactness of the callus increased,and its water content decreased.The optimum subculture medium for callus was MS +2,4-D 2.0 mg/L.The plant differentiation rate of callus in MS medium with 2.0 mg/L 6-BA and 0.10 mg/L NAA was the highest,reaching 80.2%.The regenerated plants were transplanted into 1/2 MS medium without plant growth regulator for proliferation and strong seedling,and then they were transplanted into sandy loam and their transplantation survival rate could reach 90%.Conclusion] This study established the efficient regeneration system of F.arundinacea.