| 酒精耐受型酿酒酵母菌的诱变筛选 |
| |
| 引用本文： | 易弋,黎娅,容元平,李凯.酒精耐受型酿酒酵母菌的诱变筛选[J].安徽农业科学,2009,37(34). |
| |
| 作者姓名： | 易弋 黎娅 容元平 李凯 |
| |
| 作者单位： | 广西工学院,广西柳州,545006;四川省中医药高等专科学校,四川绵阳,621000 |
| |
| 基金项目： | 广西教育厅科研项目，广西工学院博士基金项目 |
| |
| 摘 要： | 目的]筛选酒精耐受型酿酒酵母菌最佳菌株。方法]利用紫外线对酿酒酵母JL2008进行诱变处理,并结合氯化三苯四氮唑(TTC)进行筛选,对5株对酒精具有较强耐受性的突变株SC1～SC5的酒精耐受性进行比较,选择出2株酒精耐受性较强的突变株(SC1和SC5)进行酒精发酵,测定其生长速率和发酵性能。结果]2株菌的酒精耐受能力远远高于出发菌株,均能在酒精体积分数为9%的培养基上生长;在含糖15%的培养基中,SC1的酒精发酵终浓度和发酵效率与原始菌株大致相同;在含糖30%的培养基中,SC1的酒精发酵终浓度和发酵效率分别是16.54%和85.2%,均高于原始菌株。结论]突变株SC1在含酒精培养基中的生长和在浓醪条件下的发酵效果优于JL2008,是一株极具潜力的应用于浓醪发酵的酵母菌株。
|
| 关 键 词： | 酒精耐受性 酿酒酵母 诱变 筛选 |
Mutation Screening of Saccharomyces cerevisiae Strains with Alcohol Tolerance |
| |
| YI Yi et al.Mutation Screening of Saccharomyces cerevisiae Strains with Alcohol Tolerance[J].Journal of Anhui Agricultural Sciences,2009,37(34). |
| |
| Authors: | YI Yi |
| |
| Institution: | YI Yi et al (Guangxi University of Technology,Liuzhou,Guangxi 545006) |
| |
| Abstract: | Objective] The purpose was to screen out the optimum strain from Saccharomyces cerevisiae with alcohol tolerance.Method] S.cerevisiae JL2008 was mutated by ultraviolet rays and screened out with TTC.Five mutant strains with strong alcohol tolerance(SC1-SC5)were compared.Two mutant strains with the strongest alcohol tolerance(SC1 and SC5)were selected from these 5 mutant strains for alcohol fermentation,the growth rate and fermentation performance of SC1 and SC5 were determined.Result] The alcohol toleran... |
| |
| Keywords: | Alcohol tolerance Saccharomyces cerevisiae Mutation Screening |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
