银杏叶黄酮的微波提取及其抗氧化性研究

目的]研究银杏叶黄酮类物质的微波提取工艺，测定银杏叶黄酮的体外抗氧化能力，为大规模提取、开发黄酮类化合物及综合利用银杏叶资源提供依据。方法]采用微波法提取银杏叶黄酮，单因素试验考察乙醇浓度、料液比、辐射时间、回流温度对银杏叶黄酮提取率的影响。试验测试银杏叶黄酮对超氧阴离子、羟自由基清除作用。结果]微波提取的影响因素顺序为乙醇浓度〉料液比〉辐射时间〉回流温度。银杏叶黄酮对超氧阴离子、羟自由基均有较强的清除作用，且随着黄酮添加量的增大而增强。结论]银杏黄酮微波提取的最佳条件为乙醇浓度50％，料液比1：25，回流温度70℃，微波时间120s，该条件下总黄酮提取率为11．02％。银杏叶黄酮具有良好的抗氧化作用，是一种天然有效的抗氧化剂。

Research on the Flavonoids Extracted from Ginkgo Leaf with Microwave and its Antioxidant

Objective] The basis of the large-scale extraction and development of the flavonoids from ginkgo leaf and its integrated utilization was provided through the study on the microwave extraction process of ginkgo leaf flavonoids and the determination of its antioxidant capacity in vitro.Method] The effect of the concentration of ethanol,the ratio of material to liquid,the radiation time and the refluxing temperature on microwave extraction process of ginkgo leaf flavonoids was researched with single factor e...