| 微波消解分子荧光法测定茶叶中硒的含量 |
| |
| 引用本文： | 周跃花,杨菊香,王艳.微波消解分子荧光法测定茶叶中硒的含量[J].安徽农业科学,2009,37(22):10351-10353. |
| |
| 作者姓名： | 周跃花 杨菊香 王艳 |
| |
| 作者单位： | 西安文理学院化学系,陕西西安,710065;西安文理学院化学系,陕西西安,710065;西安文理学院化学系,陕西西安,710065 |
| |
| 基金项目： | 西安文理学院科研基金 |
| |
| 摘 要： | 目的]探讨微波消解分子荧光法测定茶叶中硒含量的最佳条件。方法]采用微波消解法消解茶叶样品，利用茶叶中的硒（Ⅳ）与DAN（2，3-二氨基萘）在酸性条件下反应生成具有强荧光特性的4，5一苯并苤硒脑，用荧光光度法测定茶叶中硒含量，优化微波法消解奈件。结果]采用微波化学反应器消解茶叶样品的条件为：0．5g样品加5．0ml5．0％硫酸润湿，加入HNO3：HClO4（4：1）10．0ml，在O．20kW时消解30min。最佳测定条件为：pH值1．5～2．0，EDTA．盐酸羟胺混合液用量20．0m1，0．1％DAN加入量5．0ml，沸水浴加热5min，6．0ml环己烷萃取，于激发波长377nm、发射波长519nm条件下测定荧光强度。该方法在硒含量1．0～7．0μg时，与荧光强度呈良好的线性关系，R2值为0．9909，RSD值为2．5％。结论]与湿法消解法相比，微波消解方法测定结果可靠，简单快捷。
|
| 关 键 词： | 微波消解 分子荧光法 硒 富硒茶 |
Determination on Selenium Content in Tea by Microwave Digestion and Molecule Fluorescence Photometry |
| |
| ZHOU Yue-hua et al.Determination on Selenium Content in Tea by Microwave Digestion and Molecule Fluorescence Photometry[J].Journal of Anhui Agricultural Sciences,2009,37(22):10351-10353. |
| |
| Authors: | ZHOU Yue-hua |
| |
| Institution: | ZHOU Yue-hua et al(Department of Chemistry,Xi\'an University of Arts , Science,Xian,Shaanxi 710065) |
| |
| Abstract: | Objective] The purpose was to discuss the optimum condition for determining the selenium content in tea by microwave digestion and molecule fluorescence photometry.Method] The tea sample was digested by microwave digestion.The 4,5-benzo piaselenol with the strong fluorescence characteristic was prepared by reaction between the selenium(IV) in the tea and DAN(2,3-diaminonaphthalene) under acidic condition,and then the selenium content in the tea was detected by molecule fluorescence photometry and the micr... |
| |
| Keywords: | Microwave digestion Molecule fluorescence photometry Selenium Se-enriched tea |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
