羊耳菊挥发油化学成分及其清除自由基的作用研究

目的]研究羊耳菊挥发油的化学成分及其对自由基的清除作用。方法]采用水蒸汽蒸馏法提取羊耳菊的挥发油,利用气相色谱-质谱联用法对该挥发油的化学成分进行分离鉴定,利用Fenton反应产生羟基自由基(.OH)及邻苯三酚自氧化产生超氧阴离子自由基(O.2-),以比色法测定羊耳菊挥发油对这2种自由基的清除作用。结果]挥发油得率为0.486%(V/W),确定了28种化合物的结构和相对含量。挥发油主要含3-甲基-5-异丙基-甲基氨基甲酸酚酯(74.37%)、11,14-二十碳二烯酸乙酯(9.50%)和2,2-二甲基丙酸-2-1,1-二甲基乙基]酚酯(7.59%)3种酯类化合物。挥发油对羟基自由基(.OH)和超氧阴离子自由基(O.2-)都有一定的清除能力,但清除羟基自由基(.OH)的能力较强。结论]通过对羊耳菊挥发油化学成分的研究,为进一步开发利用该植物提供了科学依据。

Chemical Composition of the Essential Oil from Inula cappa and Their Effects on Free Radicals Scavenging

Objective]The essential oils extracted from Inula cappa by steam distillation were analyzed by GC-MS.Method]Essential oil from Inula cappa extracted by steam distillation,chemical compositions of essential oil were isolated and identificated by GC-MS method.Hydroxyl radical(·OH)produced by Fenton reaction and superoxide anion radical(O·-2)produced by pyrogallol autoxidation,the scavenging effects of essential oil from Inula cappa to two kinds of free radicals determined by colorimetric method.Result]Extracting rate of essential oil from Inula cappa was 0.486%(V/W),structure of 20 kinds of compounds and their relative contents were confirmed.Essential oil mainly contented three main ester compounds of Phenol,3-methyl-5-1-methylethyl]-,methylcarbamate(74.37%),11,14-eicosadienoic acid,methyl ester(9.50%),Propanoic acid,2,2-dimethyl-,2-1,1-dimethylethyl]phenylester(7.59%).Essential oil had a certain scavenging activity on hydroxyl radical(·OH)and superoxide anion radical(O·-2),and had a better scavenging activity on hydroxyl radical(·OH). Conclusion ]Through the study on the chemical composition of essential oil from Innla cappa,which provides scientific basis for the further development and utilization of this plant.