花生四烯酸高产突变株5L罐发酵及菌丝老化研究

目的]研究花生四烯酸高产突变株的发酵及菌丝老化特性。方法]对1株经诱变获得的花生四烯酸高产突变株A1．13进行5L罐发酵试验，考察不同培养条件对发酵产物的影响。结果]碳源一次性过量加入并改变培养温度，可使该菌株花生四烯酸产量提高至602．99mg／L；发酵菌丝体在4℃条件下老化15d后，花生四烯酸产量达885．16mg/L。大豆油、花生油、玉米油、葵花籽油和芝麻油5种植物油均可起到消泡作用，添加浓度4％葵花籽油效果最佳，与对照相比，菌丝生物量和花生四烯酸产量分别提高了124％和22％。结论]该研究可为突变株A1．13工业化发酵产花生四烯酸提供理论依据和现实参考。

Study on Fermentation of High Arachidonic Acid-producing Mutant in 5 L Fermenter and Mycelium Aging

Objective]The research aimed to study the fermentation of high arachidonic acid-producing mutant and the mycelium aging.Method]Fermentation of high arachidonic acid-producing mutant A1.13 was taken in 5 L fermenter.And the products under different culture conditions was investigated.Result]The results showed that arachidonic acid (AA) yield could reach 602.99 mg/L in the batch fermentation process when large amount of carbon source was added and culture temperature were altered.After aging 15 d at 4 ℃, AA yield reached 885.16 mg/L.Five kinds of plant oils (soybean oil, arachis oil, maize oil, sunflower seed oil, sesame oil) could all be used as antifoam.Compared with the CK treatment,concentration 4% sunflower seed oil significantly increased the biomass and the AA yield by 124% and 22% respectively.Conclusion]This study could provide theoretical basis and realistic reference for the production of AA with mutant A1.13 by industrial fermentation.