呋喃西林代谢物酶联免疫检测方法的建立

目的]建立动物性食品中呋喃西林代谢物ELISA检测方法。方法]通过制备特异性强的单克隆抗体,采用间接竞争ELISA法建立动物性食品中呋喃西林代谢物ELISA检测方法,并研制出对动物性食品中呋喃西林代谢物残留检测的试剂盒。结果]该试剂盒标准曲线的IC50值为0.267 7μg/L,最低检测限为0.1μg/kg,样本加标回收率范围为79.5%～95.6%,变异系数为7.6%～9.7%。在交叉反应试验中,对呋喃西林的交叉反应率为25%,与其他药物的交叉反应率均小于1%,表明该方法具有很强的特异性。结论]该方法灵敏度、准确度、精密度均较高,能满足兽药残留的检测要求,且检测时间短(45 min),样本前处理简单、检测成本低,适合于大量样本中呋喃西林代谢物残留检测的快速筛选。

Establishment of Detection Method for the Residues of Nitrofurazone Metabolite by Enzyme-Linked Immunosorbent Assay

Objective] The research aimed to establish an ELISA method for detecting nitrofurazone metabolites in animal food.Method] Monoclonal antibodies with high specificity were prepared.And an ELISA method for detecting nitrofurazone metabolites in animal food was established by indirect competitive ELISA.The kit for detecting the residues of nitrofurazone metabolites in animal food was developed.Result] IC50 of the standard curve for the kit was 0.267 7 μg/L,the lowest detection limit was 0.1 μg/kg,the addition recovery was 79.5%-95.6% and the coefficients of variation ranged from 7.6% to 9.7%.In the cross reactivity test,the cross reactivity rate of nitrofurazone was 25% and its cross reactivity rate with other drugs was less than 1%,which indicated that this method had higher specificity.Conclusion] This method had higher sensitivity,accuracy and precision and could meet the detection demands of residues in veterinary medicine.This method had the characteristics of short detection time(45 min),simple pre-treatment,low cost and it was suitable for rapid screening for detecting the residues of nitrofurazone metabolites in a large amount of samples.