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低能Ar~+注入对玉米花粉萌发及Ca~(2+)浓度分布的影响研究
引用本文:邓方,程备久,朱苏文.低能Ar~+注入对玉米花粉萌发及Ca~(2+)浓度分布的影响研究[J].安徽农业科学,2008,36(34).
作者姓名:邓方  程备久  朱苏文
作者单位:1. 巢湖学院,安徽巢湖,238000
2. 安徽农业大学生命科学院,安徽合肥,230036
摘    要:目的]研究低能离子注入对花粉萌发及萌发过程中游离Ca2+的影响。方法]利用能量为30 keV,剂量为0.78×1015~13×1015ion/cm2的Ar+注入玉米花粉粒,研究注入后玉米花粉的萌发情况和萌发过程中游离Ca2+的分布情况。结果]当Ar+注入剂量为5.2×1015ions/cm2时,玉米花粉的萌发率有了明显的提高;而注入剂量超过7.8×1015ion/cm2以后,萌发率呈急剧下降趋势;同时利用低温孵育法在完整的玉米花粉粒中,载入酯化形式的Ca2+荧光探针fluo-3 AM后发现,花粉细胞中游离Ca2+浓度变化与花粉萌发活性变化相一致。结论]低能离子注入后引起的花粉内Ca2+浓度的动态变化可能是花粉萌发变化的初始效应。

关 键 词:Ar+  玉米  花粉萌发  Ca2+  浓度分布

Effects of Low Energy Argon Ion Implantation on Maize Pollen Germination and the Cytosolic Ca2+ Distribution during Pollen Germination Process
Abstract:Objective] The purpose of this study is to investigate the effect of low energy ion implantation on maize pollen germination and cytosolic Ca2+ distribution during pollen germination process.Method] The Ar+ with energy of 30 keV,dose of 0.78×1015~13×1015 ion/cm2,was implanted into maize pollen by bombardment,and the germination of pollen and cytosolic Ca2+ distribution during pollen germination process of the Ar+ implanted pollen were studied.Result] When been bombarded with argon ions with dose of 5.2×1015 ion/cm2,the germination rate of maize pollen increased remarkably,while implantation of ions with dose exceeding 7.8×1015 ion/cm2 sharply decreased the germination rate of maize pollen.Meanwhile,tracing of esterified Ca2+ fluorescent probe fluo-3 AM in intact pollen showed that variation of cytosolic Ca2+ concentration was consistent with the change of pollen fertility.Conclusion] The dynamics of cytosolic Ca2+ concentration caused by low energy ion implantation may be concluded as the initial effects of pollen germination.
Keywords:Ar+  Maize  Pollen germination  Ca2+ distribution
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