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实时荧光PCR法检测致敏原鱼成分的研究
引用本文:董薇,曹际娟,邱驰,吴元华.实时荧光PCR法检测致敏原鱼成分的研究[J].安徽农业科学,2009,37(23):10911-10912.
作者姓名:董薇  曹际娟  邱驰  吴元华
作者单位:沈阳农业大学,辽宁沈阳,110044;辽宁出入境检验检疫局,辽宁大连,116000;辽宁出入境检验检疫局,辽宁大连,116000;沈阳农业大学,辽宁沈阳,110044
基金项目:国家公益性行业科研专项课题 
摘    要:探讨了采用实时荧光PCR技术检测食品中的致敏原鱼成分。结果表明:采用鱼管家基因16SrRNA基因的特异性检测引物和探针进行检测时,19种样品经实时荧光PCR检测,只有鱼类样品产生阳性荧光信号,其余样品均不产生荧光信号。灵敏度试验结果表明:该方法对致敏原鱼成分的检测灵敏度较高,可达到1mg/kg,符合痕量检测要求。

关 键 词:  实时荧光PCR  致敏原检测

Study on the Detection of Allergen Fish Components in Food by Real-time Fluorescence PCR
DONG Wei et al.Study on the Detection of Allergen Fish Components in Food by Real-time Fluorescence PCR[J].Journal of Anhui Agricultural Sciences,2009,37(23):10911-10912.
Authors:DONG Wei
Institution:DONG Wei et al(Shenyang Agricultural University,Shenyang,Liaoning 110044)
Abstract:The detection of allergen fish components in food by using real-time fluorescence PCR was studied.The experiment results showed that the primers and probe of 16S rRNA gene of fish could specifically identify three kinds of fish with 19 kinds of samples.Sensitivity experiment results showed that 1 mg/kg for fish of food could be detected by using this method and the detection sensitivity was high.This method could meet the demands of trace amount detection.
Keywords:Fish  Real-time fluorescence PCR  Allergen detection  
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