| 杜仲愈伤组织诱导与继代培养研究 |
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| 引用本文： | 罗丽,赵德刚.杜仲愈伤组织诱导与继代培养研究[J].安徽农业科学,2009,37(7):2861-2863. |
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| 作者姓名： | 罗丽 赵德刚 |
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| 作者单位： | 连云港师范高等专科学校自然科学系,江苏连云港,222006;江苏省连云港市应用生物技术重点实验室,江苏连云港,222006;贵州大学农业生物工程重点实验室,贵州贵阳,550025 |
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| 基金项目： | 教育部优秀青年教师资助计划项目; 贵州省优秀教育科技人才省长专项基金(黔科合字[2001]3-2) |
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| 摘 要： | 目的]对杜仲进行愈伤组织诱导及继代培养,建立愈伤组织的培养系统。方法]以杜仲子叶、幼叶、下胚轴为外植体,接种于不同培养基中诱导和继代。结果]不同外植体愈伤组织的诱导率高低顺序为：下胚轴〉子叶〉幼叶。子叶和幼叶在MS＋NAA 1.00 mg/L、MS＋2,4-D 1.00 mg/L培养基中诱导出的愈伤组织在MS＋6-BA 1.00 mg/L＋NAA 2.00 mg/L、MS＋2,4-D 1.00 mg/L培养基中继代培养为佳;胚轴在MS＋NAA 3.00 mg/L、MS＋6-BA 1.00 mg/L＋NAA 1.00 mg/L和MS＋2,4-D 1.00 mg/L培养基中诱导出的愈伤组织在MS＋6-BA1.00 mg/L＋NAA 2.00 mg/L、MS＋6-BA 1.00 mg/L＋NAA 1.00 mg/L和MS＋2,4-D 1.00 mg/L培养基中继代培养为佳。结论]杜仲的子叶、幼叶和胚轴可采用多条途径实现愈伤组织的诱导和继代增殖培养,为进一步筛选高产细胞系打下基础。
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| 关 键 词： | 杜仲 愈伤组织 继代培养 |
Study on Callus Induction and Subculture of Eucommia ulmoides Oliv. |
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| LUO Li et al.Study on Callus Induction and Subculture of Eucommia ulmoides Oliv.[J].Journal of Anhui Agricultural Sciences,2009,37(7):2861-2863. |
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| Authors: | LUO Li |
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| Institution: | LUO Li et al(Department of Nature Science,Lianyungang Teacher\'s College,Lianyungang,Jiangsu 222006) |
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| Abstract: | Objective] The research aimed to study the callus induction and subculture of Eucommia ulmoides Oliv.to establish the callus culture system.Method] The hypocotyl,cotyledon and young leaf of E.ulmoides Oliv.were chose as explants to inoculate in a series of media with hormone at various concentrations.Result] Different explants of E.ulmoides Oliv.could all be induced into callus under suitable hormones.The induction rate in hypocotyl was higher than that of in cotyledon and young leaf.It was best that cal... |
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| Keywords: | Eucommia ulmoides Oliv Callus Subculture |
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