黄瓜种子脂肪酸含量与耐低温性关系的研究

目的]探讨黄瓜种子脂肪酸含量、种类与耐低温性的关系。方法]对15份不同来源的黄瓜材料进行白天12℃,晚上8℃的低温处理,每天光照7.5 h,处理14 d,对黄瓜的耐寒性进行分级,并计算耐寒指数,测定供试黄瓜种子的含油量、脂肪酸成分及含量。结果]黄瓜种子的脂肪酸主要有棕榈酸(16∶0)、棕榈烯酸(16∶1)、硬脂酸(18∶0)、油酸(18∶1)、亚油酸(18∶2)和亚麻酸(18∶3),与其耐寒指数进行相关分析发现,耐寒指数与亚油酸、亚麻酸含量、种子含油量I、UFA(不饱和度)达显著正相关,与棕榈酸含量、棕榈烯酸含量、硬脂酸含量相关系数很小,各材料的油酸含量基本相同。结论]黄瓜的亚油酸、亚麻酸、种子含油量I、UFA与黄瓜的耐低温性有密切的关系。

Study on the Relationship between the Contents of Fatty Acids and Low Temperature Tolerance in Cucumber Seeds

Objective] The purpose was to discuss the relationship between the contents and kinds of fatty acids and low temperature tolerance in cucumber seeds.Method] Fifteen cucumber materials from different sources were treated for 14 d with low temperature of 12 ℃ in day and 8 ℃ in night and illumination for 7.5 h per day.The cold tolerances of cucumber were graded,the cold tolerance indices were calculated out and the oil content,fatty acid composition and content in the tested cucumber seeds were determined.Result] The fatty acids in cucumber seeds mainly included 6 kinds of palmitic acid(16∶0),palmitoleic acid(16∶1),stearic acid(18∶0),oleic acid(18∶1),linoleic acid(18∶2) and linolenic acid(18∶3),etc.It was found through correlation analysis on them and the cold tolerance index that the cold tolerance index reached significantly positive correlation with the contents of linoleic acid and linolenic acid,oil content in seed,IUFA(index of unsaturated fatty acid),had very small correlation coefficient with the contents of palmitic acid,palmitoleic acid and stearic acid and the contents of oleic acid in various materials were basically same.Conclusion] The linoleic acid and linolenic acid,oil content in seed and IUFA of cucumber had close relationship with its low temperature tolerance.