微量元素对平菇菌丝生长及酶活性的影响

目的]通过添加微量元素促进平菇菌丝的生长,并富集和转化微量元素。方法]采用固体平板和液体摇瓶培养的方法培养平菇,通过测定菌丝干重、胞内多糖、过氧化物酶活性和菌丝长势等研究Zn2＋、Mn2＋、Cu2＋、Mo（VI）4种元素对平菇-126菌丝生长的影响。结果]Mo（VI）在试验所选的4种浓度范围内都促进菌丝的伸长,其他3种元素对菌丝的日伸长有不同程度的抑制作用;培养基中添加20 mg/L Zn2＋能促进菌丝分枝生长,从而提高菌丝干重;4种微量元素的加入均提高胞外蛋白的含量,其提高幅度为Mn2＋〉Cu2＋〉Mo（VI）〉Zn2＋;除Cu2＋外,其他3种微量元素在低浓度能提高发酵液过氧化物酶活性;平菇胞内多糖含量的高低与菌丝干重的量之间存在正相关性。结论]低浓度微量元素能促进平菇菌丝的生长,高浓度则抑制平菇菌丝的生长。

Influence of the Adition of Trace Element in Medium on the Pleurotus ostreatus Hphal Growth and Enzyme Activity

Objective]The accumulation of micro-element and its transformation into the Pleurotus ostreatus hphal were researched through the experiment in its addition in medium affecting hphal growth.Method]The Pleurotus ostreatus was cultured with two methods of solid medium in flat dish and liquid medium in shaking flask and the effect of 4 elements:Zn2+,Mn2+,Cu2+ and Mo(VI),on Pleurotus ostreatus variety-Pingu-126 hyphal growth was analyzed through the measurement of the dry weight of mycelium,intracellular poly...