| MAE-分光光度法测定大白口蘑中总黄酮含量 |
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| 引用本文： | 刘鸿高,王元忠.MAE-分光光度法测定大白口蘑中总黄酮含量[J].安徽农业科学,2009,37(31):15105-15107. |
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| 作者姓名： | 刘鸿高 王元忠 |
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| 作者单位： | 云南农业大学,云南昆明,650201;云南省农业科学院药用植物研究所,云南昆明,650223 |
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| 基金项目： | 教育部科学技术研究重点项目，云南省自然科学基金项目 |
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| 摘 要： | 目的]采用微波辅助萃取（MAE）法提取、以分光光度法测定大白口蘑样品中的总黄酮含量。方法]通过单因素试验和正交试验考察乙醇浓度、液固比、微波功率、微波时间等因素对微波辅助萃取法提取大白口蘑黄酮提取率的影响。对微波法与乙醇浸提法、超声波萃取法进行了比较。结果]微波萃取法最佳提取条件：乙醇浓度60％、液固比40：1、微波功率255W、微波时间5min。最优条件下，精密度和回收率试验所得结果的相对标准偏差为1．24％（n=6），回收率在98．1％～103．7％。微波法优于乙醇浸提法和超声波萃取法。结论]MAE-分光光度法简单、可靠，重现性好，稳定性好，可广泛用于各种食用茵中黄酮类物质含量的测定。
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| 关 键 词： | 微波辅助萃取 分光光度法 大白口磨 黄酮 |
Content Determination of Total Flavones from Tricholoma giganteum by Microwave-assisted Extraction and Spectrophotometry |
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| LIU Hong-gao et al.Content Determination of Total Flavones from Tricholoma giganteum by Microwave-assisted Extraction and Spectrophotometry[J].Journal of Anhui Agricultural Sciences,2009,37(31):15105-15107. |
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| Authors: | LIU Hong-gao |
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| Institution: | LIU Hong-gao et al(Yunnan Agricultural University,Kunming,Yunnan 650201) |
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| Abstract: | Objective] The research aimed to determine the content of total flavones from Tricholoma giganteum by microwave-assisted extraction and spectrophotometry.Method] The effects of solvent concentration,ratio of solution to solid,microwave power and radiation time on the extraction rate of flavone from T.giganteum by microwave-assisted extraction were studied by single factor and orthogonal experiment.The microwave method,ethanol extraction method and ultrasonic extraction method were compared.Result]The ext... |
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| Keywords: | Microwave-assisted extraction(MAE) Spectrophotometry Tricholoma giganteum Flavones |
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