猪圆环病毒2型衣壳蛋白的表达

目的]检测猪圆环病毒2型（PCV2）核衣壳蛋白（Cap）的表达,为进一步研制PCV2单克隆抗体检测抗原提供参考依据。方法]根据已经发表的PCV2的衣壳蛋白基因（Cap）设计1对引物,利用PCR方法从已知PCV2病毒中扩增到1条DNA片段。将PCR产物按预定的阅读框架插入表达载体质粒pET28a（＋）中,获得重组质粒pCAP-PCV2,并转化到大肠杆菌BL21（DE3）中。结果]序列测定表明,所克隆的序列大小为579 bp,与DQ104422的Cap基因序列一致;通过对菌体裂解物的SDS-PAGE分析证明,携带重组质粒pET-PCV2-Cap的大肠杆菌可以表达可溶性的衣壳蛋白。结论]PCV2衣壳蛋白能通过表达载体质粒pET28a（＋）进行可溶性表达。

Expression of Capsid Protein in Porcine Circovirus Type 2

Objective] To detect the expression of nucleocapsid protein(Cap) Porcine circovirus type 2(PCV2),and provide a reference for the further development of antigen monoclonal antibodies in PCV2.Method] One DNA fragment of Cap gene in PCV2 was amplified repectively by PCR using specific primers.After the DNA fragments was inserted reading frame pET28a(+) plasmid expressing carrier,Recombinant plasmid pCAP-PCV2 was transformed into Escherichia coli BL21(DE3).Result] The sequence size was 579 bp and shared 100% identity with that of DQ104422.SDS-PAGE and Western blot analysis showed that the soluble cap protein was expressed.Conclusion] PCV2 cap proteins can be expressed by the pET28a(+) carrier.