中性蛋白酶酶解绿豆蛋白制备寡肽的研究

目的]利用中性蛋白酶将绿豆蛋白质水解成肽和氨基酸,通过优化酶解条件得到水解度较高的绿豆肽。方法]选用中性蛋白酶对绿豆分离蛋白进行酶法水解以制备寡肽。在单因素考察的基础上,采用四因素三水平的正交试验设计优化酶解条件。结果]试验得出,最适的反应条件为温度50℃、pH值6.5、底物浓度7%、酶浓度6000 U/g、水解时间240 min。结论]绿豆分离蛋白在此条件下酶解,水解度的平均值可达28.82%。

Research on the Preparation of Oligopeptides with Green Bean Protein Treated by Neutral Protease

Objective] The green bean protein was hydrolyzed into peptide and amino acid with the neutral protease and the green bean peptide highly hydrolyzed was produced based on the optimal hydrolysis enzymatic condition.Method] The isolated green bean protein was hydrolyzed with neutral protease to prepare oligopeptide.The orthogonal design with four factors and three levels was applied to optimize condition based on the study single factor.Results] The result indicated that the optimal reaction condition was the temperature,50 ℃;pH value,6.5; the concentration of substrate,7%;the concentration of enzyme,6 000 U/g and the time of hydrolysis time,240 min.Conclusion] Under these conditions,average degree of hydrolysis of isolated green bean protein was up to 28.82%.