| 有机磷农药三唑磷人工抗原合成及鉴定 |
| |
| 引用本文： | 黄魁英,雷红涛,孙远明.有机磷农药三唑磷人工抗原合成及鉴定[J].安徽农业科学,2011,39(32):19843-19844. |
| |
| 作者姓名： | 黄魁英 雷红涛 孙远明 |
| |
| 作者单位： | 1. 华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州510642;广东省广州市微生物研究所,广东广州510663
2. 华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州510642 |
| |
| 摘 要： | 目的]合成并鉴定有机磷农药三唑磷人工抗原。方法]采用活泼酯法将三唑磷半抗原（TZPM-Hap）与牛血清蛋白（BSA）和卵血清蛋白（OVA）偶联,制备出免疫抗原（TZPM-A-BSA）和包被抗原（TZPM-A-OVA）,并通过紫外扫描和动物免疫试验对其进行了鉴定。结果]紫外扫描和动物免疫试验结果表明人工抗原合成成功。结论]为制备单抗及开发快速、简便的三唑磷ELISA试剂盒奠定了基础。
|
| 关 键 词： | 三唑磷 活泼酯法 人工抗原 |
Synthesis and Identification of Organophosphate Insceticide Triazophos Artificial Antigen |
| |
| Institution: | HUANG Kui-ying et al(Key Laboratory of Food Quality and Safety of Guangdong Province,College of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642) |
| |
| Abstract: | Objective] The aim was to synthesize and identify an organophosphate insceticide triazophos artificial antigen.Method] Artificial antigens for organophosphate insecticide triazophos including one immunogen and one coating antigen were prepared.The hapten(TZPM-Hap) of triazophos was coupled to bovine serum albumin(BSA) by the active ester method to prepare the artificial immunogen TZPM-A-BSA.Coupling of TZPM-Hap to ovalbumin(OVA) by the active ester method produced the coating antigen TZPM-A-OVA.The artificial antigen was identified by ultraviolet scanning and immunizing animal experiment.Result] Immunogen and coating antigen were prepared successfully and confirmed by ultraviolet spectrometry and immunizing animal.Conclusion] The research lays a basis for the preparation of monoclonal antibody and the development of quick and simple triazophos ELISA kits. |
| |
| Keywords: | Triazophos Active ester method Artificial antigen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
