| 红姬凤梨离体培养植株再生关键技术研究 |
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| 引用本文： | 孟艳琼,张志平,何琬璐,傅松玲.红姬凤梨离体培养植株再生关键技术研究[J].安徽农业科学,2009,37(12):5364-5366. |
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| 作者姓名： | 孟艳琼 张志平 何琬璐 傅松玲 |
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| 作者单位： | 安徽农业大学林学与园林学院,安徽合肥,230036;安徽农业大学园艺学院,安徽合肥,230036 |
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| 基金项目： | 安徽省教育厅青年教师基金项目(2004JQ153); 安徽农业大学校长青年基金(校科字[2005]9号) |
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| 摘 要： | 目的]建立红姬凤梨组织培养快繁体系。方法]以MS培养基为基本培养基添加植物生长调节剂，对红姬凤梨叶片进行愈伤组织诱导，并进行芽分化、芽的继代增殖和生根培养。结果]叶鞘愈伤组织诱导以MS＋2，4-D0．5mg／L＋BA3．0mg／L＋KT2．0mg／L培养基最好、愈伤组织诱导率和分化率分别达82．4％和40．2％；丛生芽诱导以MS＋2，4-D0．2MG/L＋NAA0．05mg／L＋BA3．0mg／L＋KT2．0mg／L培养基增殖效果最佳，增殖倍数迭9．72；生根诱导以1／2MS＋NAA3．0mg/L培养基诱导生根效果最好，平均每株生根12．12条。结论]采用组织培养可有效去除病毒，增加繁殖系数。
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| 关 键 词： | 红姬凤梨 叶片培养 诱导 植株再生 |
Key Technologies in vitro Plant Regeneration of Tissue Culture of Cryptanthus bromelioides |
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| MENG Yan-qiong et al.Key Technologies in vitro Plant Regeneration of Tissue Culture of Cryptanthus bromelioides[J].Journal of Anhui Agricultural Sciences,2009,37(12):5364-5366. |
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| Authors: | MENG Yan-qiong |
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| Institution: | MENG Yan-qiong et al(College of Forestry , Gardening,Anhui Agricultural University,Hefei,Anhui 230036) |
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| Abstract: | Objective] The research aimed to establish rapid propagation system of Cryptanthus bromelioides'Tricolor'.Method] The leaves of Cryptanthus bromelioides'Tricolor' were carried on callus induction,bud differentiation,bud subculture proliferation and rooting culture with the MS culture medium added hormones.Result] The results showed that MS medium supplemented with 2,4-D 0.5 mg/L,BA 3.0 mg/L,KT 2.0 mg/L was best for callus induction in which the induction rate and differentiation rate of callus were 82.4%... |
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| Keywords: | Cryptanthus bromelioides \'Tricolor\' Leaf culture Induction Plant regeneration |
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