| 耐酸性α-淀粉酶产生菌的发酵条件优化 |
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| 引用本文： | 刘雅琴,乌日娜,段金华.耐酸性α-淀粉酶产生菌的发酵条件优化[J].安徽农业科学,2010,38(35):19888-19890. |
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| 作者姓名： | 刘雅琴 乌日娜 段金华 |
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| 作者单位： | 北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021 |
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| 基金项目： | 国家民委发酵酿造工程生物技术重点实验室资助项目 |
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| 摘 要： | 目的]优化耐酸性α-淀粉酶产生菌的发酵条件。方法]在筛选出的耐酸性α-淀粉酶产生菌的基础上,对其培养基C、N含量、接种龄、接种量、初始pH值、摇瓶转速及温度等发酵条件进行优化。结果]耐酸性α-淀粉酶产生菌最佳发酵条件为接种龄14 h,接种量8%,初始pH值5.5,发酵温度35℃,转速150 r/min,接种菌液量25 ml,培养基中C、N的含量分别为1.0%。结论]在优化条件下,酶活力达到31.4 U/ml,比未优化时提高了65.3%。
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| 关 键 词： | α-淀粉酶 酶活力 发酵 优化 |
Optimization of the Fermentation Condition for Acid-resistant α-amylase Producing Strain |
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| Institution: | LIU Ya-qin et al(School of Bioscience and Engineering,The North University for Ethnics,Yinchuan,Ningxia 750021) |
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| Abstract: | Objective] The aim was to optimize the fermentation conditions of the acid-resistant α-amylase producing strain.Method] Based on the selected acid-resistant α-amylase producing strain,the C and N content in its medium,inoculum age,inoculum volume,initial pH,shaking speed of flask,temperature and other fermentation conditions were optimized.Result] The best optimization conditions for the acid-resistant α-amylase producing strain were 14 h of inoculum age,8% of inoculum volume,5.5 of initial pH,35℃ of the fermentation temperature,150 r/min of shaking speed,25 ml of inoculum bacteria liquid as well as 1.0% of C and 0.5% of N in the medium.Conclusion] Under the optimized conditions,the enzyme activity could reach 31.4 U/ml,65.3% more than that before optimization. |
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| Keywords: | α-amylase Enzyme activity Fermentation Optimization |
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