安祖花组织培养和植株再生的研究

目的]为安祖花幼苗的规模化生产提供依据。方法]以叶片、叶柄以及组培苗气生根为外植体,进行安祖花愈伤组织的诱导,探索安祖花再生植株规模化生产的最佳途径。结果]叶片、叶柄以及组培苗气生根均能成功地诱导产生愈伤组织,其中叶片诱导率最高(92%),组培苗气生根诱导率可达82%。诱导愈伤组织分化产生不定芽的最佳培养基为1/2MS+6-BA1.0 mg/L+KT0.1 mg/L。培养基1/2 MS+IBA0.5 mg/L和MS+IBA0.5 mg/L均可诱导不定芽产生根,无明显差异。将珍珠沙和花泥按1∶1(V∶V)混合后作为组培苗移栽的培养基质,经30 d培养,幼苗成活率达91%。结论]以气生根为外植体,规模化再生植株只需55~60 d,生产成本也大大降低。

Study on the Tissue Culture and Plant Regeneration of Anthurium andraeanum Lind

Objective] The research aimed to provide basis for large-scale production of Anthurium andraeanum Lind.seedlings.Method] With leaf blade,petiole and aerial root in tissue culture seedling as explants, the callus induction of A.andraeanum was carried out and the optimum approach for the large-scale production of the regenerated plants in A.andraeanum were discussed.Result] Leaf blade,petiole and aerial root of tissue culture seedling could all successfully induce the production of callus,among which the stimulate rate of leaf blade was highest(92%) and that of the aerial root of tissue culture seedling could reach 82%.The optimum medium for inducing the adventitious bud differentiation from callus was 1/2 MS+6-BA 1.0 mg/L+KT 0.1 mg/L.Both medium 1/2 MS+IBA 0.5 mg/L and medium MS+IBA 0.5 mg/L could induction root production from the adventitious buds without obvious differences.When the mixture of perlite and flower foam(by the volume ratio of 1∶1)was taken as the culture substrate for the transplantation of tissue culture seedlings,the survival rate of A.andraeanum seedlings reached 91%.Conclusion] With the aerial roots as explants,the plant regeneration in a large scale only needed 55～60 d and the production cost was also greatly decreased.