几株微生物产絮凝活性比较及培养条件优化

目的]对比几株微生物产絮凝活性及不同培养条件对絮凝活性的影响。方法J取几种不同的微生物菌种采用高岭土悬浊液法分别测其絮凝率，选择一种絮凝率高的菌种分别改变培养基的成分以及助凝剂等条件，优化其产絮凝剂的最佳参数。结果]枯草芽孢杆菌在30℃、170r／min下培养72h后，其絮凝率最高，达95．7％。不同的碳源对絮凝剂产生的影响不同，从高到低为葡萄糖〉蔗糖〉淀粉〉乙醇〉甘油，以25g/L葡萄糖为碳源，发酵液的絮凝率高达91．4％。氮源以复合性氮源为佳，絮凝率〉80．0％。培养基pH值为7．5，絮凝效果最佳。助凝剂以添加1．8mmol／L的Ca^2＋效果最佳。结论]不同菌株不同培养条件产生的絮凝活性不同。

Study on the Comparison of Flocculent Activity and the Optimize of Culture Conditions of Several Strains Microorganisms

Object] Comparison of production of flocculent activity of several strains and influence of culture condition on flocculent activity. Method] Several strains microorganisms were chose, the flocculent rates were measured by aqueous suspension of kaolin method, and highest flocculent rate was chose to change the component of medium and coagulant aid and so on , optimized the optimum parameters of its produce flocculant . Result] The flocculent rate of Bacillus subtilis reached 95.7% at cultural condition of 30 ℃,170 r/min, 72 h. Different carbon sources had different effects on the flocculent rate, the rates as follow, glucose> sucrose> starch> ethanol> glycerol. 25 g/L glucose as a carbon source, the flocculent rate of fermentation broth reached 91.4%. The nitrogen compound was better than simple nitrogen, the flocculent rate > 80% . The best effect of flocculent was pH value 7.5 of medium. coagulant aid added 1.8mmol/L Ca~(2+). Conclusion] Different strains and cultural condition had different flocculent activities.