Cd·Zn复合污染对蚕豆叶绿体含量的影响

目的]研究Cd、Zn复合污染环境中蚕豆叶绿素含量的变化情况。方法]在培养液中设计Cd的浓度为0、0.1、1.0、10.0、50.0mg/L,Zn的浓度为0、5、50、100、200 mg/L处理蚕豆苗,处理后第10天取豆苗相同叶位的叶片,研究Cd、Zn单一及复合污染对蚕豆叶绿素含量的影响。结果]单一Cd、Zn处理的蚕豆叶绿素含量都低于对照,且随着浓度的增加,叶绿素含量降低。除0.1 mg/L Cd+5 mg/LZn处理外,其余Cd、Zn复合污染处理的蚕豆叶绿素含量都低于相应的单一处理。在单一Cd各处理中加入Zn,随着Zn浓度的增加,蚕豆叶绿素含量下降的趋势更加明显,说明Zn增强了Cd对蚕豆的毒害作用,显示出协同作用。结论]Cd、Zn的共存状态对蚕豆的毒害有协同、促进和加和作用,不容忽视。

Effects of Cd and Zn Combined Pollution on Chlorophyll Content in Broad Bean

Objective] The study was to research the change of chlorophyll content in broad bean in the environment of combined pollution by Cd and Zn.Method] Cd concn.of 0,0.1,1,10 and 50 mg/L and Zn concn.of 0,5,50,100 and 200 mg/L in culture fluid were designed to treat the seedlings of broad bean.On the 10th day after treatment,the leaves on the same leaf position of bean seeding were taken to study the effect of single pollution by Cd or Zn and combined pollution by Cd and Zn on chlorophyll content in broad bean.Result] The chlorophyll contents in broad bean in the treatment of single pollution by Cd or Zn were all lower than that of CK,and with the increasing of Cd or Zn concn.the chlorophyll content was decreased.Except the treatment with 0.1 mg/L Cd+ 5 mg/L Zn,the chlorophyll content in broad bean in other treatments of combined pollution by Cd and Zn were all lower than that in the treatment of corresponding single pollution.When Zn was added in each treatment of single pollution by Cd,with the increasing of Zn concn.,the downward trend of chlorophyll content in broad bean was more obvious,which indicated that Zn increased the poison effect of Cd on broad bean,Zn and Cd showed synergistic effect.Conclusion] The poison of coexisting status of Cd and Zn had?cooperative,promotive and additive action,and this action was unnegligible.