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金银花提取液抑菌活性的研究
引用本文:康旭,李冬生,邓川,袁江兰.金银花提取液抑菌活性的研究[J].安徽农业科学,2010,38(27):14935-14936.
作者姓名:康旭  李冬生  邓川  袁江兰
作者单位:湖北工业大学生物工程学院,发酵工程省部共建教育部重点实验室,湖北武汉,430068;湖北工业大学生物工程学院,发酵工程省部共建教育部重点实验室,湖北武汉,430068;湖北工业大学生物工程学院,发酵工程省部共建教育部重点实验室,湖北武汉,430068;湖北工业大学生物工程学院,发酵工程省部共建教育部重点实验室,湖北武汉,430068
摘    要:目的]对金银花水提液和醇提液的抑菌活性进行研究。方法]首先用滤纸片法测定了样品对几种受试菌的抑制活性,再采用微量稀释法测定了敏感菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果]金银花水提液对金黄色葡萄球菌和枯草芽孢杆菌具有抑制作用,对金黄色葡萄球菌的M1C为19.25%,MBC为38.50%;对枯草芽孢杆菌的MIC为38.50%。金银花醇提液对沙门氏菌、金黄色葡萄球菌、巨大芽孢杆菌具有抑制作用,对沙门氏菌的MIC为9.80%,MBC为19.60%;对金黄色葡萄球菌MIC为19.60%,MBC为39.20%;对巨大芽孢杆菌的MIC为19.60%。结论]金银花水提液和醇提液对部分受试菌具有明显的抑制作用。

关 键 词:金银花  水提液  醇提液  最小抑菌浓度  最小杀菌浓度

Study on Antimicrobial Activity of Extracts from Lonicera japonica Thunb
Institution:KANG Xu et al ( Key Laboratory of Fermentation Engineering, Ministry of Education, College of Bioengineering, Hubei University of Technology, Wuhan, Hubei 430068 )
Abstract:Objective] Antimicrobial activity of extract by water and alcohol from Lonicera japonica Thunb was investigated.Method] Firstly,filter paper method was applied to detect inhibitory effect of the samples on tested strains,and then the minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were obtained by micro dilution method.Result] Water extract from Lonicera japonica Thunb inhibited Staphyloccocus aureus and Bacillus subtilis.MIC and MBC on Staphyloccocus aureus was 19.25% and 38.50% respectively,and MIC on Bacillus subtilis was 38.50%.Alcohol extract had some inhibition on Salmonella,Staphyloccocus aureus and Bacillus megaterium.MIC and MBC on Salmonella was 9.80% and 19.60% respectively,MIC and MBC on Staphyloccocus aureus was 19.60% and 39.20% respectively,and MIC on Bacillus megaterium was 19.60%.Conclusion] Extract by water and alcohol from Lonicera japonica Thunb inhibited some of tested strains obviously.
Keywords:Lonicera japonica Thunb  Water extract  Alcohol extract  Minimum inhibitory concentration(MIC)  Minimum bactericidal concentration(MBC)
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