| 一种用于转基因水稻快速检测的DNA提取方法 |
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| 引用本文： | 苟小清,付振艳,王晓军.一种用于转基因水稻快速检测的DNA提取方法[J].安徽农业科学,2012,40(35):17014-17015,17018. |
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| 作者姓名： | 苟小清 付振艳 王晓军 |
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| 作者单位： | 中国科学院新疆理化技术研究所,新疆乌鲁木齐830011;中国科学院研究生院,北京100049;中国科学院新疆理化技术研究所,新疆乌鲁木齐,830011 |
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| 基金项目： | 中国科学院"西部博士"资助项目，新疆维吾尔自治区自然基金会项目 |
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| 摘 要： | 目的]建立一种快速筛选转基因水稻的DNA提取方法。方法]对试剂盒法和快速法2种DNA提取方法进行比较,并分别用转Ta NADP-ME1和Ta NADP-ME2基因的转基因水稻进行验证。结果]用快速法提取得到的DNA进行PCR扩增,可分别得到约1 700 bp的Ta NADP-ME2基因和约1 900 bp的Ta NADP-ME1基因,且条带清晰明亮,与试剂盒法相比无显著差异。结论]该研究中快速提取DNA的方法经济、简便、快捷,适合用于对大量转基因样品进行检测。
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| 关 键 词： | 转基因水稻 DNA快速提取 PCR模板 |
A Rapid DNA Extraction Method for Detection of Transgenic Rice |
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| Institution: | GOU Xiao-qing et al(The Xinjiang Technical Institute of Phaysics & Chemistry.CAS,Urumqi,Xinjiang 830011) |
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| Abstract: | Objective] To establish a rapid DNA extraction method for the screening of transgenic rice.Method] The kit method and fast method of two DNA extraction methods were compared,and were proved by Ta NADP-ME1 and Ta NADP-ME2 gene transgenic rice respectively.Result] PCR was done by using the DNA which extracted by the rapid method as template,about 1 700 bp Ta NADP-ME2 gene and about 1 900 bp Ta NADP-ME1 gene were obtained respectively,and the strips were clear and bright,comparing with the kit method showed no significant difference.Conclusion] The rapid DNA extraction method of this study is economic,simple and quick,suitable for the large transgenic samples detection. |
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| Keywords: | Transgenic rice Rapid DNA extraction PCR |
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