| 兴安落叶松DNA提取-试剂盒改良法 |
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| 引用本文： | 王洪梅,李艳霞,翁海龙,周显昌,康迎昆,王福德,王鑫.兴安落叶松DNA提取-试剂盒改良法[J].安徽农业科学,2012,40(35):17029-17030,17041. |
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| 作者姓名： | 王洪梅 李艳霞 翁海龙 周显昌 康迎昆 王福德 王鑫 |
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| 作者单位： | 黑龙江省林业科学研究所,黑龙江哈尔滨,150081 |
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| 基金项目： | 国家林业公益性行业科研专项子项目 |
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| 摘 要： | 目的]寻找一种快速简便的兴安落叶松基因组DNA的提取方法。方法]对DNA提取试剂盒进行改良,与原试剂盒法(离心柱)进行比较,并用琼脂糖凝胶电泳、蛋白核酸测定和ISSR-PCR对所提取的总DNA进行检测。结果]试剂盒改良法提取的总DNA纯度和浓度较高,电泳显示其主条带较清晰,无降解现象,A260/A280值为1.75～1.84。结论]改良后的DNA提取方法更适合兴安落叶松基因组DNA的提取。
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| 关 键 词： | 兴安落叶松 DNA提取 基因组DNA 提取试剂盒 改良法 |
Extracted Larix gmelinii DNA with Improved DNA Extraction Kits |
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| Institution: | WANG Hong-mei et al(Forestry Research Institute of Heilongjiang Province,Harbin,Heilongjiang 150081) |
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| Abstract: | Objective] To develop a quick and convenient method for the extraction of genomic DNA of Larix gmelinii.Method] Improved DNA extraction kits were compared with the original kit method(spin columns),and extracted total DNA were detected by agarose gel electrophoresis,protein and nucleic acid determination and ISSR.Result] The purity and concentration of total DNA was higher extracted by improved DNA extraction kits.The electrophoresis showed that the main bands are clearer and no degradation phenomena.A260/A280 value was 1.75 to 1.84.Conclusion] The optimal genomic DNA extraction of L.gmelinii was obtained using improved DNA extraction kits method. |
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| Keywords: | Larix gmelinii DNA extraction Genomic DNA Extraction kits Improved method |
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