| 卡瓦胡椒离体再生体系的建立 |
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| 引用本文： | 李明芳,郑学勤.卡瓦胡椒离体再生体系的建立[J].安徽农业科学,2012,40(35):17005-17006,17025. |
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| 作者姓名： | 李明芳 郑学勤 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海南海口,571101;中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 摘 要： | 目的]建立卡瓦胡椒(Piper methysticum)的离体再生体系。方法]以卡瓦胡椒的带芽嫩茎和嫩叶作为外植体,通过在培养基中添加不同的激素组合,来筛选最佳再生培养基。结果]卡瓦胡椒的丛芽诱导培养基为MS+4 mg/L 6-BA+0.5 mg/L GA3+0.65%琼脂+3%蔗糖+0.012 5%PVP,最佳芽伸长根生长培养基为1/2MS+5 nmol/L JA+0.65%琼脂+3%蔗糖。结论]为卡瓦胡椒的工业化生产提供了理论指导。
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| 关 键 词： | 卡瓦胡椒 离体 再生体系 |
Establishment of Regeneration System in vitro for Piper methysticum |
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| Institution: | LI Ming-fang et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101) |
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| Abstract: | Objective] This study aimed to establish in vitro regeneration system for Piper methysticum.Method] With tender kava stems and leaves with bud as explants,different hormone combinations were added into basic medium,to screen the optimal regeneration medium.Result] The optimal induction medium for multiple buds of kava was MS + 4 mg/L 6-BA + 0.5 mg/L GA3+0.65% agar + 3% sucrose + 0.012 5% PVP;the optimal regeneration medium for kava plantlets was 1/2MS +5 nmol/L JA +0.65% agar +3% sucrose.Conclusion] This study provides theoretical guidance for the enlargement of industrial production of kava. |
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| Keywords: | Piper methysticum in vitro Regeneration system |
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