实时荧光定量PCR技术在乳品微生物学中的应用研究进展

越来越多的基因组测序给特异性引物和探针的设计带来了极大的支持。最近有研究表明,基于定量聚合酶链式反应（qPCR）方法的技术成功应用于发酵过程中的微生物计数以及乳制品中益生菌数量的计算。基于qPCR方法的技术进步包括实时荧光定量PCR技术,不仅可以计数活菌数量,还可以在单一反应体系中检测多个微生物菌群数量,且能构建高通量PCR流程。实时荧光定量PCR技术在食品工业中的应用已证明其可靠性,尽管如此,该技术在乳品工业中的广泛使用还需要更多的技术支持和标准化方法。综述了实时荧光定量PCR技术及其在乳品微生物学中的最新研究与应用进展。

Recent Advances of Real-time Quantitative PCR in Dairy Microbiology

Recent studies show that quantitative PCR（ qPCR）-based methods are applied with success in enumerating fermenting microbes and health promoting bacteria in dairy products. The increasing number of sequenced genomes is now a crucial support to designing specific primers and probes. Technological advances in qPCR-based methods including viable quantitative PCR are also highlighted and could be promising strategies for quantifying merely viable cells,detecting multiple microorganisms in a single reaction and constructing high throughput PCR workflows. Application of real-time quantitative PCR in the food industry has proven its reliability nevertheless its widespread use in the dairy industry will need technical recommendations and standardization methods. In this paper,we proposed to synthesized major outputs from recent studies using PCR to display how this can offer new analytical solutions to dairy microbiology.