猪繁殖与呼吸综合征病毒宁夏地区地方株的分离与鉴定

目的]弄清宁夏地区部分猪场出现“无名高热”病因及更加有效预防、控制此类疾病的发生.方法]通过从宁夏某发病猪场采集产生典型病变的猪肺部组织,将病料处理后分别用Marc-145、PK-15、BHK-21等传代细胞系进行病毒的分离与鉴定.结果]只有Marc-145细胞上有典型病变,连续传代3次后病变稳定,接毒48 ～72 h后病变率达70％左右.RT-PCR及双酶切的结果表明分离培养物为猪繁殖与呼吸综合征病毒毒株.通过RNA提取、RT-PCR及酶切反应,实现了对分离培养物快速、确切鉴定.结论]用MARC-145细胞成功分离到1株PRRSV,命名为ZH-w株.

Isolation and Identification of Porcine Reproductive and Respiratory Syndrome Virus Strain from Ningxia Area

Objective ] The research aimed to understand the causes of unknown fever in some pig farms of Ningxia area and effectively pre- vent and control the occurrence of this disease. Method] The typical lung tissues of diseased pigs were collected from some pig farm of Ningx- ia. And the collected samples were treated with Marc-145, PK-15 and BHK-21 and other continuous cell lines to make the virus isolation and identification. Result] Only Mare-145 cell lines showed clear CPE. And it was stable after 3 passages of virus and the disease rate reached 70% after 48 - 72 h. The results of RT-PCR and double enzyme digestion showed the isolated strain was a strain of porcine reproductive and respiratory syndrome virus. The isolated strains were rapidly and accurately identified by RNA extraction, RT-PCR and double enzyme diges- tion. Conclusion] A strain of PRRSV was successfully separated and named as ZH-w.