金银花中总黄酮的提取及其抗氧化活性的研究

目的]对金银花总黄酮抗氧化性进行研究。方法]采用醇提法提取金银花中的总黄酮,利用烘箱法测定其抗氧化性、H2O2/Fe2+体系法测定其对羟基的清除作用、邻苯三酚法测定其对超氧阴离子的清除作用。结果]金银花醇提物对猪油的氧化具有明显的抑制作用;对羟基的清除达52.59%;对自由基的清除达17.12%。结论]总黄酮具有明显的抗氧化、清除羟自由基以及清除超氧阴离子的能力。

Extraction of Total Flavonoids from FLOS LONICERAE JAPONICAE and Its Oxidation Resistance

Objective] To research on the oxidation resistance of total flavonoids from FLOS LONICERAE JAPONICAE.Method] Total flavonoids were extracted from FLOS LONICERAE JAPONICAE by ethanol extraction.The oxidation resistance of total flavonoids was detected by Oven Method;the scavenging effect on hydroxy was detected by H2O2/Fe2+ system method;the scavenging effect on superoxide anion was detected by pyrogallol autoxidation method.Result] Extracts from FLOS LONICERAE JAPONICAE showed significant inhibitory effects on the oxidation of lard oil;the scavenging rates on hydroxyl and free radical reached 52.59% and 17.12%,respectively.Conclusion] total flavonoids had significant antioxidant ability,hydroxyl radical scavenging activity and superoxide anion scavenging activity.