车前草的不同提取物抗菌活性比较研究

目的]研究车前草不同提取物的抗菌活性,比较提取物的抗菌能力。方法]将车前草以8种不同溶剂进行室温提取,采用分光光度比浊法测定不同提取物在96孔板中对3种细菌的抗菌活性。结果]抗菌活性最好的是车前草无水乙醇提取物(P0.05),乙醚提取物和无水甲醇提取物次之,自来水和超纯水提取物没有抗菌活性。结论]车前草的不同溶剂提取物均具有一定的抗菌活性,对金黄色葡萄球菌和大肠杆菌的抑制作用最为明显,对绿脓杆菌的抑制作用也较好。

Comparison of the Antibacterial Activity of Different Extracts from Plantago asiatica L.

Objective] The aim was to study the antibacterial activity of different extracts from Plantago asiatica L.,and compare their antibacterial capacity.Method] The Plantago asiatica L.was extracted with eight different solvents under room temperature,and the antibacterial activity of different extracts against three bacteria in the 96-wells dish was determined by spectrophotometry.Result] The best antibacterial activity was found in the anhydrous ethanol extracts(P<0.05),then followed by the ether and anhydrous methanol extracts,the extracts by running water and ultrapure water had no antibacterial activity.Conclusion] The extracts of Plantago asiatica L.with different solvents all show certain antibacterial activity,especially against Staphyloccocus aureus and Escherich coli,the inhibitive effect against P.aeruginosa was also good.