滇重楼最佳授粉期的研究

目的]优选滇重楼的最佳授粉期。方法]采用TTC法测定花粉的活力,并采用联苯胺-过氧化氢法测定柱头可授性,根据研究滇重楼花粉活力变化规律及柱头可授性优选滇重楼的最佳授粉期。结果]滇重楼花粉囊开裂时活力最高为68.2%,前2 d内,花粉活力下降不明显。第4天开始,活力明显下降,但仍然具有一定的活性。柱头的活性在花粉囊开裂后5 d内最强,滇重楼的授粉应选择在这5 d内进行。结论]该研究优选了滇重楼的最佳授粉期,为滇重楼的进一步开发利用提供了依据。

Preliminary Study on the Optimal Pollination Period of Paris polyphylla var.yunnanensis

Objective] To select the optimal pollination period of Paris polyphylla var.yunnanensis.Method] The pollen viability of P.polyphylla was evaluated by TTC test(2,3,5-triphenyl tetrazolium chloride) and its stigma receptivity was estimated by benzidine-H2O2 method.Result] Pollen viability was the highest at 68.2% when pollen sac cracked.It did not decrease significantly in the first 2 days.Since the fourth day,the pollen viability declined obviously,but still remained some activity.The stigma receptivity was in high activity within five days after pollen sac cracking and the pollination of P.polyphylla should be done in this period.Conclusion] The optimal pollination period of P.polyphylla is selected in this experiment,which can provide basis for further development and utilization of P.polyphylla.