| 应用正交试验法优选茎瘤芥高效遗传转化体系 |
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| 引用本文： | 梁婷,刘毅,蔡应繁,岳显可,江怀仲,何晓红.应用正交试验法优选茎瘤芥高效遗传转化体系[J].安徽农业科学,2012,40(14):8031-8034. |
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| 作者姓名： | 梁婷 刘毅 蔡应繁 岳显可 江怀仲 何晓红 |
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| 作者单位： | 成都中医药大学研究生院,四川成都,611130;重庆邮电大学生物信息学院,四川重庆,400065 |
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| 基金项目： | 国家自然科学基金，重庆市自然科学基金 |
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| 摘 要： | 目的]建立茎瘤芥高效遗传转化体系,为茎瘤芥及芸薹属植物遗传转化奠定基础。方法]应用正交试验法研究茎瘤芥遗传转化过程中预培养时间、共培养时间、侵染液浓度、侵染时间、对转化率的影响。结果]最适合茎瘤芥CRY2基因RNAi载体遗传转化的条件为预培养3 d、共培养4 d、侵染液浓度OD600=0.5、侵染10 min,此时Kana选择压为30 mg/L。结论]优选出了农杆菌转化的最宜条件,遗传转化率有较大提高。
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| 关 键 词： | 茎瘤芥 正变法 遗传转化 |
Optimization of Genetic Transformation System of Tumorous Stem Mastard by Orthogonal Design |
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| Institution: | LIANG Ting et al(Graduate School,Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 611130) |
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| Abstract: | Objective]To establish the genetic transformation system,so as to lay foundation for the genetic transformation of tumorous stem mustard and other Brassica plants.Method]The effects of different time of preculture,coculture,bacteria liquid concentration and dipping time on the transformation rate of tumorous stem mustard were studied with orthogonal design.Result] The best conditions for genetic transformation were 3 d preculture,4 d coculture,bacteria liquid concentration(OD600) 0.5 and 10 min dipping,then the Kan concentration was 30 mg/L.Conclution]The optimum conditions for the transformation of tumorous stem mustard were selected,which greatly improved the transformation rate. |
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| Keywords: | Tumorous Stem mustard Orthogonal design Genetic transformation |
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