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| 林蛙皮活性肽抗氧化活性研究 | |
| 引用本文： | 周涌,张岚,李杰,高硕阳,刘彦麟,刘雅娟.林蛙皮活性肽抗氧化活性研究[J].安徽农业科学,2012,40(14):8080-8082,8098. |
| 作者姓名： | 周涌 张岚 李杰 高硕阳 刘彦麟 刘雅娟 |
| 作者单位： | 1. 吉林医药学院公共卫生系,吉林吉林,132013 2. 吉林医药学院公共卫生系,吉林吉林132013;吉林农业大学食品科学与工程学院,吉林长春130118; 3. 吉林大学公共卫生学院,吉林长春,130120 |
| 摘 要： | 目的]探讨林蛙皮活性肽的抗氧化活性。方法]林蛙皮经木瓜蛋白酶水解后,将产物经葡聚糖凝胶G-25层析纯化,测定活性组分对DPPH.、.OH、O2-.的清除能力。结果]柱层析共分离出4个组分(PⅠ、PⅡ、PⅢ、PⅣ),各组分抗氧化能力的大小顺序依次为PⅡPⅢPⅠPⅣ。PⅡ组分对各自由基清除能力的大小顺序依次为DPPH..OHO2-.。蛋白浓度为1 mg/ml时,对DPPH.的清除率为94.23%;蛋白浓度为16 mg/ml时,对.OH的清除率为86.79%,对O2-.的清除率仅为26.32%。结论]该研究为林蛙的综合开发和利用提供了新的依据。 |
| 关 键 词： | 林蛙皮 活性肽 抗氧化活性 |
Study on Antioxidant Activity of Active Peptide from Skin of Forest Frog(Rana chensinensis) |
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| Institution: | ZHOU Yong et al(Department of Public Health,Jilin Medical College,Jilin,Jilin 132013) |
| Abstract: | Objective] The aim was to study the antioxidant activity of active peptide from skin of forest frog.Method] The papain hydrolysate of skin of forest frog was purified by Sephadex G-25 gel filtration,and the scavenging activity of DPPH·、·OH and O-2· of active components was determined.Result] The results showed that there were 4 components were obtained,the antioxidant activities were in the order:PⅡ>PⅢ>PⅠ>PⅣ.The order of radical scavenging activity of fraction PⅡ was DPPH·>·OH> O-2·.The scavenging rate of DPPH· was 94.23% when the concentration of the protein was 1 mg/ml,and the scavenging rate of ·OH and O-2· were 86.79% and 26.32% respectively when the concentration of the protein was 16 mg/ml.Conclusion] The study provides a new basis for the comprehensive development and utilization of forest frog. |
| Keywords: | Skin of forest frog Active peptide Antioxidant acti vity |
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