野生小花碧冬茄外植体器官分化与植株再生研究

目的]为野生小花碧冬茄组织快繁技术提供依据,文中研究了组培条件下,不同浓度的6-BA、NAAI、BA处理对野生小花碧冬茄外植体器官分化和植株再生的影响。方法]以野生小花碧冬茄无菌种子获得的试管苗为材料,筛选其成苗培养和生根培养最适激素组合。结果]成苗培养中,较高浓度的6-BA配合较低浓度的NAA有利于外植体形成丛生芽,而高水平的NAA会引起愈伤组织的大量产生。对野生小花碧冬茄而言,芽分化最佳培养基组合为MS+蔗糖30 g/L+琼脂粉5 g/L+NAA 0.2 mg/L+6-BA 1.5 mg/L;愈伤组织最佳培养基组合为MS+蔗糖30 g/L+琼脂粉5 g/L+NAA 0.4 mg/L+6-BA 1.0 mg/L;生根最佳培养基组合为1/2MS+蔗糖30 g/L+琼脂粉5 g/L+IBA 1.0 mg/L+NAA 0.4 mg/L。结论]明确野生小花碧冬茄芽分化、愈伤组织和生根培养的理想激素配比,初步建立了野生小花碧冬茄的高效再生体系。

Study on Organ Differentiation and Plantlet Regeneration from Explants of Wild Petunia Juss

Objective] This study aimed to provide the basis for rapid propagation technology of wild Petunia Juss..Method] Effects of different plant hormones 6-BA,NAA and IBA on the organ differentiation and plantlet regeneration of wild Petunia Juss..explants were investigated.The optimal medium for seedling culture and rooting induction were selected with aseptic plantlets of wild Petunia Juss..Result] Higher concentration of 6-BA in combination with lower concentration of NAA is beneficial to the formation of multiple-bud clumps induced by callus,and a higher level of NAA will lead to a sharp increase of callus.The optimal medium for bud differentiation is MS+30 g/L of sucrose+5 g/L of agar+0.2 mg/L of NAA+1.5 mg/L of 6-BA;the optimal medium for callus culture is MS+30 g/L of sucrose+5 g/L of agar+0.4 mg/L of NAA+1.0 mg/L of 6-BA;the optimal medium for rooting induction is 1/2MS+30 g/L of sucrose+5 g/L of agar+1.0 mg/L of IBA+0.4 mg/L of NAA.Conclusion] The optimal exogenous hormone combinations for bud regeneration,callus culture and rooting induction were defined,an efficient regeneration system of wild Petunia Juss..was established preliminarily.