兽医临床致病性大肠杆菌超广谱β-内酰胺酶的检测 |
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引用本文: | 张春辉,张晓根,唐桂芬.兽医临床致病性大肠杆菌超广谱β-内酰胺酶的检测[J].安徽农业科学,2008,36(30). |
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作者姓名: | 张春辉 张晓根 唐桂芬 |
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作者单位: | 郑州牧业工程高等专科学校,河南郑州,450011;西北农林科技大学,陕西杨凌,712100;郑州牧业工程高等专科学校,河南郑州,450011 |
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基金项目: | 河南省教育厅资助项目
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河南省科技厅资助项目
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摘 要: | 目的]指导临床合理用药,控制兽医临床的感染性疾病。方法]用分子生物学方法对用双纸片协同法检测产生ESBLs的5株猪鸡致病性大肠杆菌进行了ESBLs的检测。结果]产生ESBLs的猪鸡致病性大肠杆菌中都扩增出了TEM型引物。结论]双纸片协同法检测ESBLs具有较高的可靠性,兽医临床大肠杆菌已经产生ESBLs。
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关 键 词: | 超广谱β-内酰胺酶 大肠杆菌 耐药性 检测 |
Detection of ESBLs in Veterinary Inclinical Pathogenicity Escherichia coli |
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Abstract: | Objective] The aim of this paper was to instruct the clinical rational drug use,control veterinary inclinical infectious diseases.Method] Molecular biological method was used to conduct the ESBLs detection on 5 strains of swine and avian pathogenic E.coli,which could produce ESBLs under the detection of double-disk method.Result] TEM primer was amplified from the swine and avian pathogenic E.coli,which could produce ESBLs.Conclusion] The double-disk method for ESBLs detection was highly reliable.Veterinary inclinical E.coli could produce ESBLs. |
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Keywords: | ESBLs Escherichia coli Drug resistance Detection |
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