RP-HPLC-UVD法测定莽吉柿中α-倒捻子素的含量

目的]建立一种测定莽吉柿（Garcinia mangostana L.）中α-倒捻子素（alpha-mangostin）含量的方法。方法]采用反相高效液相色谱-紫外检测法（RP-HPLC-UVD）测定。色谱条件：迪马公司DiamonsilTMC18色谱柱（250.0mm×4.6mm,5.0μm）;流动相,甲醇-水（83∶17）;流速,1.0ml/min;柱温,25℃;紫外检测波长,317nm。结果]在选定的色谱条件下,反相高效液相色谱-紫外检测法符合精密度、准确度和线性（R^2〉0.999）的基本要求,加样回收率为98.9%,RSD为1.87%。3批莽吉柿样品中α-倒捻子素含量分别为36.4、37.3、38.5mg/g,平均含量为37.4mg/g。结论]该方法简便,准确,灵敏度高,重现性好,可用于莽吉柿中α-倒捻子素含量的测定。

Determination of Alpha-mangostin in Pericarpium Garciniae mangostanae L.by RP-HPLC-UVD

Objective] To establish a method for determination of alpha-mangostin in Garcinia mangostana L..Method] Reverse phase high-performance liquid chromatography (RP-HPLC) method with a ultra-violet detector (UVD) was performed on a 5 μm DiamonsilTM C18 column (250.0 mm × 4.6 mm) at 25 ℃,the mobile phase composition was methanol-water (83∶17),the flow rate was at 11.0 ml/min, the detection wavelength was 317 nm.Result] The method fulfilled all the standard requirements of precision, accuracy and linearity (R2>0.999).The recovery was 98.9%, RSD was 1.87%.Alpha-mangostin contents of three batches samples of Garcinia mangostana L.were 36.4, 37.3, 38.5 mg/g respectively, the average content was 37.4 mg/g.Conclusion] RP-HPLC-UVD method is simple, accurate, high sensitivity, good reproducibility, and can be used for determination of alpha-mangostin in Garcinia mangostana L..