金属离子对悬浮培养霍山石斛类原球茎合成活性多糖的影响

采用单因素试验和正交试验,考察了Mg2+、Ca2+、Fe2+、Mn2+和Zn2+5种金属离子对悬浮培养霍山石斛类原球茎合成活性多糖的影响。结果表明:1.5 mmol/L Mg2+4、.5 mmol/L Ca2+0、.1 mmol/L Fe2+0、.5 mmol/L Mn2+和0.06 mmol/L Zn2+的组合最有利于类原球茎多糖的合成,在此组合下多糖产量达最高(5.87 g/L),比优化前提高2.49倍,比标准MS培养基提高31倍,且5种离子对类原球茎合成多糖影响的主次顺序为Mg2+>Ca2+>Mn2+>Fe2+>Zn2+。

Effect of Metal Ion on the Polysaccharide Synthesis in Protocormlike Body of Dendrobium huoshanense in Suspension Culture

This research is concerned with the effect of Mg~(2 ),Ca~(2 ),Fe~(2 ),Mn~(2 )and Zn~(2 ) on polysaccharide synthesis in Protocorm-like body of Dendrobium huoshanense in suspension culture through the orthogonal matrix method with one factor-at-a-time.The results showed that the optimal combination for polysaccharide accumulation in protocorm-like body was 1.5 mmol/L Mg~(2 ),4.5 mmol/ L Ca~(2 ),0.1 mmol/L Fe~(2 ),0.5 mmol/L Mn~(2 ) and 0.06 mmol/L Zn~(2 ).The peak of the polysaccharide production(5.87 g/L) was obtained in the culture medium with the same ion combination mentioned above,which was increased 2.49-fold than that of the former experiment and was increased 31-fold than that obtained in standard MS.Furthermore,the responsible order of metal ion in the polysaccharide synthesis was Mg~(2 )> Ca~(2 )> Mn~(2 )> Fe~(2 )> Zn~(2 ).