| 棉花茎尖农杆菌转化体系的建立 |
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| 引用本文： | 赵福永.棉花茎尖农杆菌转化体系的建立[J].安徽农业科学,2009(2):515-518. |
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| 作者姓名： | 赵福永 |
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| 作者单位： | 长江大学生命科学学院,湖北荆州,434025 |
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| 基金项目： | 长江大学博士科研启动基金项目 |
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| 摘 要： | 目的]建立高效的以草甘膦为选择剂的棉花茎尖农杆菌遗传转化体系。方法]以中棉35号、中棉27号、石远321和珂字312号4个陆地棉品种茎尖为遗传转化受体材料，采用农杆菌介导转化法，结合草甘膦筛选，探讨了影响棉花茎尖遗传转化效率的几个重要因素。结果]棉花茎尖对草甘膦极为敏感，所有品种棉花茎尖均在60μmol／L草甘膦浓度下停止生长。对茎尖用OD600=1．0菌液浸染20min、预培养2d、恢复培养1周和共培养72h的处理能显著提高棉花遗传转化效率。对所有成活转基因棉株进行PCR检测的结果表明，所有植株均能扩增出目的基因aroA—M1 1．3kh的特定条带。结论]该研究为进一步加速转基因棉花的产业化进程奠定基础。
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| 关 键 词： | 棉花 茎尖 农杆菌 草甘膦 遗传转化 |
Establishment of Agrobacterium-mediated Transformation System of Cotton Shoot Tip |
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| ZHAO Fu-yong.Establishment of Agrobacterium-mediated Transformation System of Cotton Shoot Tip[J].Journal of Anhui Agricultural Sciences,2009(2):515-518. |
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| Authors: | ZHAO Fu-yong |
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| Institution: | ZHAO Fu-yong(College of Life Science,Yangtze University,Jingzhou,Hubei 434025) |
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| Abstract: | Objective] The study aimed to establish Agrobacterium-mediated transformation system of cotton(Gossypium hirsutum L.) shoot tip with glyphosate as the selective agent.Method] With the shoot tips of 4 upland cotton varieties of Zhongmian 35,Zhongmian 27,Shiyuan 321 and Coker 312 as explants,using Agrobacterium-mediated transformation methods combined with glyphosate screening,some key factors that affected transformation frequency of cotton shoot tip were discussed.Result] The cotton shoot tips were sensi... |
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| Keywords: | Cotton Shoot tip Agrobacterium Glyphosate Genetic transformation |
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