| 植物内生细菌对利福平的抗性及紫外诱变研究 |
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| 引用本文： | 刘佳佳,马竹凤,闫淑珍.植物内生细菌对利福平的抗性及紫外诱变研究[J].安徽农业科学,2010,38(18):9580-9581. |
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| 作者姓名： | 刘佳佳 马竹凤 闫淑珍 |
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| 作者单位： | 南京师范大学生命科学学院,江苏南京,210046 |
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| 基金项目： | 基础科学人才培养基金 |
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| 摘 要： | 目的]从植物体内分离筛选内生细菌,了解其在植物体内的定殖动态。方法]选用5株植物内生细菌H1、DP1、CJL1、DJL12和YC1作为出发菌株,在明确其对利福平的抗性水平后,对抗性较低的H1菌株和DLJ12菌株进行紫外诱变试验。结果]经初筛,5种植物内生菌在不含利福平的平板上生长良好,其中H1、DLJ12在浓度10μg/ml的利福平下均不能生存,其他3个菌种均能在浓度50μg/ml的利福平下生存。诱导后得到了抗利福平浓度达到80μg/ml的DLJ12菌株。结论]该研究可为开发利用内生细菌提供理论和实践依据。
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| 关 键 词： | 植物内生细菌 紫外诱变 利福平 |
Study on Determination of Endophytic Bacteria Resistance to Rifampin and Its UV-mutagenesis |
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| LIU Jia-jia et al.Study on Determination of Endophytic Bacteria Resistance to Rifampin and Its UV-mutagenesis[J].Journal of Anhui Agricultural Sciences,2010,38(18):9580-9581. |
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| Authors: | LIU Jia-jia |
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| Institution: | LIU Jia-jia et al(School of Life Science,Nanjing Normal University,Nanjing,Jiangsu 210046) |
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| Abstract: | Objective]The study aimed to isolate and screen the endophytic bacteria from the plant and understand their colonization dynamics in plants.Method]5 kinds of endophytic bacteria including H1,DP1,CJL1,DJL12 and YC1were selected as the original strains,after making clear their resistance level against rifampin,the UV-mutagenesis experiment was conducted on the H1 and DLJ12 strains with lower resistance.Result]Through preliminary screening,5 kinds of endophytic bacteria could grow well on the plat without r... |
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| Keywords: | Endophytic bacteria UV mutagenesis Rifampicin |
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