激光诱变对黄皮洋葱须根的生物学效应研究

目的]对激光诱变黄皮洋葱须根的生物学效应进行研究。方法]采用CO2激光和He-Ne激光对黄皮洋葱2个品种的湿种子进行辐射,通过随机区组设计,利用生物统计学和生理生化的方法,从个体及生化水平上考查激光诱变黄皮洋葱L1代须根的生物学效应。结果]经激光诱变处理的黄皮洋葱在须根长度、数目、须根重以及根系活力等方面,表现为不同处理引起的生物学效应差异显著,其中,激光种类不同引起须根长度的变异达到5%显著差异;洋葱品种不同,激光辐照引起的须根变异明显,但各处理间差异均未达到5%显著水平;激光剂量不同,引起须根数的变异达5%显著水平;激光辐射对洋葱根系活力表现为刺激效应。结论]该研究结果为激光诱变黄皮洋葱育种提供了参考依据。

A Study on the Biological Effect of Laser-induced Mutation on Onion Adventitious Roots

Objective] This study aimed to investigate the biological effect of laser-induced mutation on fibrous roots of yellow skin onion.Method] Wet seeds of two yellow skin onion cultivars were radiated by CO2 laser and He-Ne laser at three dosage levels separately.A randomized complete block design with three replications was adopted.The biological effect of laser-induced mutation on L1-generation fibrous roots of yellow skin onion was investigated with biostatistics and physiological and biochemical methods.Result] Significant variations in the biological effects caused by various laser treatments were observed in the length,quantity,fresh weight and activity of onion adventitious roots.Specifically,the variation in adventitious root length induced by different types of laser reached 5% significance level;significant variation was observed in adventitious roots of different onion cultivars induced by laser,while the variation among each treatment did not reach 5% significance level;the variation in adventitious root quantity induced by different dosage levels of laser reached 5% significance level;laser radiation showed stimulating effect on root activity of onion.Conclusion] This study provided reference for laser-induced breeding of yellow skin onion.