分批补料及缺氮培养对小球藻油脂产量的影响

目的]为了实现小球藻的高密度及高产油培养。方法]通过分析分批培养过程中藻细胞的生长曲线、葡萄糖消耗曲线、pH及溶氧变化曲线,对小球藻进行分批补料,待藻细胞达到一定的高密度后再进行缺氮培养以富集细胞内的油脂。结果]经过4次分批补料,小球藻的生物量达到了65.25 g/L,然后进行缺氮培养12 h,小球藻的油脂含量由42.75%提高到了63.82%,油脂产量达43.37 g/L。结论]合理的分批补料明显地提高了小球藻的生物量。缺氮培养进一步提高了小球藻的油脂含量。

Effects of Fed-batch Cultivation and Nitrogen Deficiency on Lipid Yield of Chlorella vulgaris

Objective] To achieve high yield of biomass and lipid of Chlorella vulgaris.Method] The growth curve of C.vulgaris,variation curves of residual glucose concentration,pH,dissolved oxygen of the medium during batch cultivation were analyzed to culture C.vulgaris accordingly by fed-batch until certain density,and then the C.vulgaris was cultured with deficient nitrogen to enrich the lipid.Result] After fed-batch cultivation for four times,the biomass yield of C.vulgaris reached 65.25 g/L,then cultured by nitrogen deficiency for another 12 hours,the lipid content was improved from 42.75% to 63.82%,and the lipid yield reached 43.37 g/L.Conclusion] Reasonable fed-batch culture greatly improves the biomass of C.vulgaris,while the cultivation by nitrogen deficiency further increases its lipid content.