铜绿微囊藻荧光对HgCl_2生物毒性响应规律的研究

目的]研究铜绿微囊藻荧光对HgCl2生物毒性响应规律。方法]以铜绿微囊藻为材料,以铜绿微囊藻在其最佳发射和发射波长处的荧光强度为测量指标,初步探讨了不同质量浓度的HgCl2对铜绿微囊藻荧光强度值的影响。结果]不同浓度的HgCl2对铜绿微囊藻有不同的影响,低浓度(0.002～0.004 mg/L)的HgCl2对铜绿微囊藻的光合作用有促进作用,表现为藻液的荧光值变小;0.010～0.400mg/L的HgCl2对铜绿微囊藻的光合作用有抑制作用,表现为藻液荧光值变大,且随着HgCl2浓度增高这种抑制作用也随之变大。可见HgCl2对铜绿微囊藻的荧光变化效果呈显著的浓度-毒性正相关关系(R2=0.963 5)。结论]该研究为快速测定水质毒性提供新的理论依据。

Study on Response Rule of Microcystis aeruginosa Fluorescence on the Biological Toxicity of HgCl2

Objective] The research aimed to study response rule of M.aeruginosa fluorescence on the biological toxicity of HgCl2.Method] M.aeruginosa as material,fluorescence intensity at its best launch and emission wavelength as measured indicator,influence of the HgCl2 at different mass concentrations on M.aeruginosa fluorescence intensity was discussed initially.Result] HgCl2 at different mass concentrations had different influences on M.aeruginosa.Low concentration(0.002-0.004 mg/L)of HgCl2 could promote photosynthesis of M.aeruginosa.It showed as fluorescence value of the algae liquid becoming smaller.0.010-0.400 mg/L of HgCl2 inhibited photosynthesis of M.aeruginosa.It showed as fluorescence value of the algae liquid becoming bigger.Moreover,inhibition effect increased as HgCl2 concentration rose,showing a positive correlation between HgCl2 concentration and toxicity(R2=0.963 5).Conclusion] The research provided new theoretical basis for quickly measuring water toxicity.