大叶胡颓子茎正丁醇萃取物的抗菌作用研究

目的]研究大叶胡颓子茎正丁醇萃取物的抑菌作用。方法]大叶胡颓子茎经乙醇提取后,用石油醚、氯仿、乙酸乙酯和正丁醇依次萃取,然后采用滤纸片法和平板稀释法对正丁醇萃取物进行体外抑菌作用研究。结果]大叶胡颓子茎正丁醇萃取物对5种常见的呼吸道感染菌(金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、肺炎克氏杆菌和鲍曼不动杆菌)均有体外抑制作用,最小抑菌浓度分别为1.500、0.750、0.375、0.750和0.375 mg/ml。结论]大叶胡颓子茎正丁醇萃取物具有抗菌活性,可以进一步开发其药用价值。

Antibacterial Effects of n-Butyl Alcohol Extract from Elaeagnus macrophylla Thunb. Stems

Objective]To study the antibacterial effects of n-butyl alcohol extract from Elaeagnus macrophylla Thunb. stems.Meathod]Elaeagnus macrophylla thunb. stems were firstly extracted by ethanol,and then extracted with petroleum ether,chloroform,ethyl acetate,n-butyl alcohol,respectively. And the extracorporeal antimicrobial effects of n-butyl alcohol was studied by filter paper and plate dilution method.Result] The n-butyl alcohol extract from Elaeagnus macrphylla Thunb. stems had extracorporeal antimicrobial effects on five common pathogenic bacteria of respiratory tract,including Staphyloccocus aureus,Escherichia coli,Pseudomonas aennosa,Klebsiella pneumoniae,Acinetobacter baumannii,and their MICs were 1. 500,0. 750,0. 375,0. 750 and 0. 375 mg / ml,respectively.Conclusion]The n-butyl alcohol extract from Elaeagnus macrophylla Thunb. stems was the active ingredient and could be exploited for medical treatment.