Ca2+和Sr2+联合处理对油菜抗氧化酶活性的影响

目的]为了了解环境介质中同时存在Ca2+和Sr2+2种离子时油菜幼苗的抗氧化酶活性的变化。方法]在石英砂和Hoagland营养液培养体系中,用浓度为0、10、20和40 mmol/L SrCl2及浓度为0、5和10 mmol/L Ca(NO3)2处理3叶龄油菜幼苗。在处理0、7、14和21 d后,分别取样测定POD、CAT和SOD的活性。结果]在相同的Sr2+浓度下,随着Ca2+浓度的增加,POD活性增加,Ca2+明显缓解Sr2+对油菜幼苗的毒害作用。结论]该研究从抗氧化角度初步阐明油菜具有较高富集Sr2+能力的机理,为利用油菜对锶污染环境进行植物修复提供理论依据。

Effects of Combined Treatment of Ca2+ and Sr2+ on Antioxidant Enzymes Activities of Oilseed Rape Seedlings

Objective] The research aimed to investigate the effects of combined treatment of Sr2+ and Ca2+ on antioxidant enzymes activities of oilseed rape seedlings.Method] Three-leaf stage oilseed rape seedlings(Brassica napus L.cv.Mianyou No.15) were raised in quartz-hydroponic culture system,and a solution containing 0,10,20 or 40 mmol/L SrCl2,and 0,5 or 10 mmol/L Ca(NO3)2 was applied.Three antioxidant enzymes(POD,CAT and SOD) activities were determined after 0,7,14 and 21 d of the combined treatment of Sr2+ and Ca2+.Result] POD,CAT and SOD activities were increased gradually with the increase of Ca2+ concentration.Ca2+ could alleviate the deleterious effect induced by Sr2+ in oilseed rape seedlings.Conclusion]The strontium accumulation capability of oilseed rape was explained from the perspective of antioxidant rapeseed,providing a theoretical basis for the use of oilseed rape phytoremediation of strontium to cleanup the polluted environments.