1株益生蜡样芽孢杆菌发酵培养基优化研究

目的]通过优化蜡样芽孢杆菌的发酵培养基,提高蜡样芽孢杆菌的生物量。方法]在初始发酵培养基的基础上,通过单因素试验和正交试验确定蜡样芽孢杆菌发酵培养基的最适碳氮源和碳氮比。结果]优化后的发酵培养基为:葡萄糖15.00 g/L,可溶性淀粉40.00 g/L,蛋白胨10.00 g/L,玉米浆干粉30.00 g/L,硫酸铵3.00 g/L,磷酸氢二钾5.00 g/L,磷酸二氢钠5.00 g/L,硫酸镁1.25 g/L,硫酸锰0.60 g/L,氯化钙2.00 g/L。优化后蜡样芽孢杆菌在50 L不锈钢发酵罐中的发酵液活菌数达1.6×1010cfu/m L,较优化前提高了1个数量级。结论]优化后的蜡样芽孢杆菌发酵培养基大大提高了该菌的生物量,为工业化放大生产提供了参考。

Optimization of Fermentation Medium for A Probiotic Bacillus cereus

Objective] To increase the biomass of Bacillus cereus by optimizing the its fermentation medium.Method] On the basis of initial fermentation medium,the single factor experiment and orthogonal experiment were used to determine the optimum carbon and nitrogen ratios in fermentation medium.Result] The optimized fermentation medium was:15.00 g/L glucose,40.00 g/L soluble starch,10.00 g/L peptone,corn steep powder 30.00 g/L,3.00 g/L ammonium sulfate,5.00 g/L dipotassium hydrogenphosphate,5.00 g/L dihydrogen phosphate sodium,1.25 g/L magnesium sulfate,0.60 g/L manganese sulfate,and 2.00 g/L calcium chloride.After optimization,fermented viable cell count of Bacillus ce-reus in 50 L cans reached up to 1.6 ×1010 cfu/mL,which improved an order of magnitude comparing with that before optimization .Conclusion] The optimized culture medium greatly enhances the biomass of Bacillus cereus,which provides references for the large-scale production in industri-alization.