| 养殖大菱鲆腹水病病原菌的分离与鉴定 |
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| 引用本文： | 景亚运,张正,王印庚,于永翔,廖梅杰,李彬.养殖大菱鲆腹水病病原菌的分离与鉴定[J].安徽农业科学,2016,44(24). |
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| 作者姓名： | 景亚运 张正 王印庚 于永翔 廖梅杰 李彬 |
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| 作者单位： | 大连海洋大学水产与生命学院，辽宁大连116023;中国水产科学研究院黄海水产研究所，青岛海洋科学与技术国家实验室，海洋渔业科学与食物产出过程功能实验室，山东青岛266071;中国水产科学研究院黄海水产研究所，青岛海洋科学与技术国家实验室，海洋渔业科学与食物产出过程功能实验室，山东青岛266071 |
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| 基金项目： | 山东省自主创新与成果转化专项(2014ZZCX06205)。 |
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| 摘 要： | 目的]从养殖大菱鲆中分离腹水病病原菌,并对其进行鉴定。方法]从山东半岛2个不同的养殖场腹水病发病大菱鲆体内分离病原菌,并通过常规生理生化试验和16S r DNA基因序列对比对其进行鉴定。结果]共分离到2株优势细菌。常规生理生化特征分析表明,这2株菌分别与鲨鱼弧菌(Vibrio carchiariae)和大菱鲆弧菌(Vibrio scophthalmi)表性特征非常相似。系统发育树分析表明这2株菌与鲨鱼弧菌(Vibrio carchiariae)和大菱鲆弧菌(Vibrio scophthalmi)的亲缘关系最近。结论]这2株细菌被鉴定为鲨鱼弧菌(Vibrio carchiariae)和大菱鲆弧菌(Vibrio scophthalmi)。
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| 关 键 词： | 大菱鲆 腹水病 病原 鲨鱼弧菌 大菱鲆弧菌 |
Isolation and Identification of Pathogenic Bacterium Associate with Ascites Disease of Cultured Scophthalmus maximus |
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| Abstract: | Objective] To isolate and identify pathogenic bacterium associate with ascites disease of cultured Scophthalmus maximus.Method] Pathogenic strains were isolated from three diseased-turbot samples associate with ascites syndrome in three different aquatic plants on Shandong Peninsula.The traditional physiological and biochemical tests and 16S rDNA sequencing analysis were performed on the two strains.Result] A total of two dominant bacterium were isolated.Analysis of traditional physiological and biochemical test showed that the two strains were Vibrio carchiariae and Vibrio scophthalmi.Analysis of phylogenetic dendrogram showed that the two strains had close genetic relationship with Vibrio carchiariae and Vibrio scophthalmi. Conclusion] The two strains were identified as Vibrio alginolyticus and Vibrio scophthalmi. |
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| Keywords: | Scophthalmus maximus Enteritis diseases Pathogeny Vibrio alginolyticus Vibrio scophthalmi |
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