云南小粒速溶咖啡中百菌清提取净化方法研究

目的]探讨云南小粒速溶咖啡中百菌清的最佳提取净化方法。方法]分别采用石油醚-丙酮萃取法、固相吸附法及二氯甲烷-丙酮萃取法这3种不同的方法提取净化云南小粒速溶咖啡中的百菌清,并利用高效液相色谱进行加标回收测定,根据回收率的大小和净化方式的繁简,确定最适宜的百菌清测定方法。结果]3种不同方法提取净化百菌清的加标回收率分别为85.3%、111.4%和118.7%。结论]通过回收率及净化方式的比较可知,可利用固相吸附法对云南小粒速溶咖啡中的百菌清进行提取纯化。

Stndy on Extraction and Purification Method of Chlorothalonil in Yunnan Small Grain Instant Coffee

Objective] The aim was to explore the optimal extraction and purijication method for chlorothalonil in Yunnan small grain instant coffee. Method] Three different extraction and purification methods ( aether petrolei-acetone extraction, solid-phase adsorption and dichlo-romethane-acetone extraction) were performed to extract chlorothalonil in instant coffee .Then all of the extractions were tested using high per-formance liquid chromatography(HPLC).The most suitable method was determined according to the percentages of recovery measurement. Result] The results obtained by three different extraction and purification methods showed that the recovery percentages were 85.3%, 111. 4% and 118.7%, respectively.Conclusions] By comparing recovery rate and purification methods, solid-phase adsorption method was the best one for testing chlorothalonil in Yunnan small grain instant coffee.