七叶一枝花种子萌发和组织培养研究 |
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引用本文: | 黄宗华.七叶一枝花种子萌发和组织培养研究[J].安徽农业科学,2016,44(27):118-121. |
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作者姓名: | 黄宗华 |
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作者单位: | 广东培正学院,广东广州,510830 |
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基金项目: | 湖北省自然科学基金项目(2010CDA035);中南民族大学植物遗传与发育创新团队资助项目。 |
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摘 要: | 目的]研究七叶一枝花种子萌发的最适赤霉素(GA3)浓度和组织培养的最适培养基。方法]采用不同浓度GA3处理种子,研究促进种子快速萌发且萌发率高的GA3浓度,再将胚根3~4 cm长的种子播于土壤中,分别在光照培养箱和自然条件下生长并观察第一片叶的长势。采用不同浓度的6-BA诱导愈伤组织,研究愈伤组织诱导的适宜6-BA浓度。结果]150 mg/L GA3处理种子的萌发率最高,种子外植体最合适的愈伤组织诱导培养基为添加0.1 mgL/6-BA和0.5 mg/L NAA的1/2MS基本培养基。结论]七叶一枝花种子的快速萌发方法是可行的。
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关 键 词: | 七叶一枝花 种子 萌发率 愈伤组织 |
Seeds Germination and Tissue Culture ofPa ris po lpy hylla Smith |
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Abstract: | Objective] To research the optimal GA3 concentration and the optimal culture medium for seed germination of Paris polyphylla Smith. Method]Seeds of P.polyphylla were processed by different concentrations of GA3 .The optimal GA3 concentration to promote the rapid seed germination and high germination rate was researched.Seeds with 3-4 cm radicle were planted in the soil.Under the condition of illumina-tion and natural growth, growth of the first leaf was observed, respectively.Callus were induced by different concentrations of 6-BA, so as to find the optimal concentration of 6-BA.Result] In 150 mg/L GA3 treatment, seeds had the highest germination rate.The optimal callus induction medium for seed explants was 1/2 MS +0.1 mg/L 6-BA +0.5 mg /L NAA.Conclusion] This ratpid germination method for P.polyphylla is feasible. |
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Keywords: | Paris polyphylla Smith Seeds Germination rate Callus |
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