| 超高压高效液相色谱法测定茶酒中茶氨酸含量 |
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| 引用本文： | 杨威,吕超志,刘峰,李丹,曹忠.超高压高效液相色谱法测定茶酒中茶氨酸含量[J].安徽农业科学,2016,44(30). |
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| 作者姓名： | 杨威 吕超志 刘峰 李丹 曹忠 |
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| 作者单位： | 长沙理工大学化学与生物工程学院，微纳生物传感与食品安全检测协同创新中心，湖南长沙410114;长沙理工大学化学与生物工程学院，微纳生物传感与食品安全检测协同创新中心，湖南长沙410114;长沙理工大学化学与生物工程学院，微纳生物传感与食品安全检测协同创新中心，湖南长沙410114;长沙理工大学化学与生物工程学院，微纳生物传感与食品安全检测协同创新中心，湖南长沙410114;长沙理工大学化学与生物工程学院，微纳生物传感与食品安全检测协同创新中心，湖南长沙410114 |
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| 基金项目： | 国家自然科学基金项目(31527803)。 |
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| 摘 要： | 目的]研究茶酒中茶氨酸含量的分析方法。方法]以邻苯二甲醛(OPA)为衍生剂,采用超高压高效液相色谱测定茶酒中的茶氨酸。结果]检测茶氨酸的线性范围为0.1~100.0μg/m L,线性方程可拟合为Y=53.57X-41.664,相关系数r为0.999 59,该方法的最低检出限达到0.05μg/g(以S/N=3计)。样品中茶氨酸的含量为0.25 mg/kg,加标试验的回收率在99.6%~100.1%。结论]该方法快速、简单、定量准确,可在茶氨酸的测定分析中推广应用。
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| 关 键 词： | 超高压 高效液相色谱 茶酒 茶氨酸 |
Determination of Theanine in Tea Liquor by Ultra High Pressure and High Performance Liquid Chromatography |
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| Abstract: | Objective] The analytical method for content of theanine in tea liquor was investigated.Method] Using o-phthalaldehyde (OPA) as a derivating agent,a method was established for determination of theanine in tea liquor by super high-pressure high performance liquid chromatog-raphy.Result] The results showed that the linear range of 0.1-100.0 μg/mL for theanine detection was obtained,and the corresponding linear equation was fitted as Y=53.57X-41.664 with correlation coefficient r of 0.999 59.Also,the minimum detection limit of 0.05 μg/g (S/N=3) was reached.The theanine content of the sample was determined as 0.25 mg/kg with a recovery rate of 99.6%-100.1%.Conclusion] The method is rapid,simple and accurate,which is easy to be widely used in the determination and analysis of theanine in tea liquor . |
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| Keywords: | Ultra high pressure High performance liquid chromatography Tea liquor Theanine |
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